Engraftment of donor T cells is not inhibited by GCV treatment
| Group . | n . | Thy1.1 (mean ± SD) . | Thy1.2 (mean ± SD) . |
|---|---|---|---|
| BM (GCV−) | 3 | 2.4 ± 1.2 | 23.4 ± 7 |
| BM (GCV+) | 2 | 4.5 ± 3.7 | 14.4 ± 5.0 |
| Tg−(GCV−) | 7 | 1.4 ± 1.3 | 10.4 ± 7.3 |
| Tg−(GCV+) | 2 | 0.7 ± 0.5 | 10.3 ± 10.8 |
| IL-2-tk (GCV−) | 2 | 0.7 ± 0.4 | 7.4 ± 5.8 |
| IL-2-tk (GCV+) | 4 | 2.1 ± 1.9 | 12.5 ± 6.5 |
| IL-4-tk (GCV−) | 2 | 3.4 ± 1.6 | 15.0 ± 0.23 |
| IL-4-tk (GCV+) | 4 | 2.4 ± 2.2 | 18.1 ± 5.1 |
| Group . | n . | Thy1.1 (mean ± SD) . | Thy1.2 (mean ± SD) . |
|---|---|---|---|
| BM (GCV−) | 3 | 2.4 ± 1.2 | 23.4 ± 7 |
| BM (GCV+) | 2 | 4.5 ± 3.7 | 14.4 ± 5.0 |
| Tg−(GCV−) | 7 | 1.4 ± 1.3 | 10.4 ± 7.3 |
| Tg−(GCV+) | 2 | 0.7 ± 0.5 | 10.3 ± 10.8 |
| IL-2-tk (GCV−) | 2 | 0.7 ± 0.4 | 7.4 ± 5.8 |
| IL-2-tk (GCV+) | 4 | 2.1 ± 1.9 | 12.5 ± 6.5 |
| IL-4-tk (GCV−) | 2 | 3.4 ± 1.6 | 15.0 ± 0.23 |
| IL-4-tk (GCV+) | 4 | 2.4 ± 2.2 | 18.1 ± 5.1 |
Spleen cells were stained with antibodies to recipient (Thy1.1) and donor (Thy1.2) Thy1 alleles and then analyzed by FACS. The percentage of donor and recipient cells among the total cell population is indicated, along with the number of mice analyzed. It is evident that GCV treatment did not adversely affect donor T-cell engraftment.