Effect of GCV treatment on T-cell subsets in spleen
| Cell population . | Experiment A . | Experiment B . | ||||||
|---|---|---|---|---|---|---|---|---|
| BM . | IL-2-tk . | BM . | IL-4-tk . | |||||
| PBS . | GCV . | PBS . | GCV . | PBS . | GCV . | PBS . | GCV . | |
| CD4 | ||||||||
| Naive | 29 ± 5.2 | 28 ± 3.3 | 19 ± 8.1 | 33 ± 6.73-150 | 32 ± 0.1 | 23 ± 3.5 | 10 ± 5.8 | 17 ± 9.3 |
| Activated | 19 ± 1.1 | 24 ± 2.2 | 9 ± 1.6 | 13 ± 2.23-150 | 26 ± 11 | 30 ± 7.4 | 27 ± 9.9 | 34 ± 6.7 |
| Memory | 40 ± 0.8 | 41 ± 2.5 | 63 ± 10.1 | 46 ± 6.63-150 | 34 ± 5.4 | 41 ± 0.2 | 59 ± 9.2 | 44 ± 7.83-151 |
| CD8 | ||||||||
| Naive | 8 ± 3.5 | 9 ± 4.5 | 8 ± 4.1 | 13 ± 4.13-152 | 20 ± 1.0 | 19 ± 3.4 | 4 ± 2.4 | 12 ± 5.83-152 |
| Activated | 28 ± 13 | 32 ± 10 | 21 ± 4.6 | 28 ± 6.83-152 | 37 ± 10 | 37 ± 8 | 22 ± 11.8 | 34 ± 8.03-152 |
| Memory | 50 ± 9.5 | 49 ± 12 | 60 ± 7.3 | 46 ± 7.63-150 | 27 ± 8.4 | 33 ± 1.8 | 61 ± 6.2 | 45 ± 8.03-151 |
| Cell population . | Experiment A . | Experiment B . | ||||||
|---|---|---|---|---|---|---|---|---|
| BM . | IL-2-tk . | BM . | IL-4-tk . | |||||
| PBS . | GCV . | PBS . | GCV . | PBS . | GCV . | PBS . | GCV . | |
| CD4 | ||||||||
| Naive | 29 ± 5.2 | 28 ± 3.3 | 19 ± 8.1 | 33 ± 6.73-150 | 32 ± 0.1 | 23 ± 3.5 | 10 ± 5.8 | 17 ± 9.3 |
| Activated | 19 ± 1.1 | 24 ± 2.2 | 9 ± 1.6 | 13 ± 2.23-150 | 26 ± 11 | 30 ± 7.4 | 27 ± 9.9 | 34 ± 6.7 |
| Memory | 40 ± 0.8 | 41 ± 2.5 | 63 ± 10.1 | 46 ± 6.63-150 | 34 ± 5.4 | 41 ± 0.2 | 59 ± 9.2 | 44 ± 7.83-151 |
| CD8 | ||||||||
| Naive | 8 ± 3.5 | 9 ± 4.5 | 8 ± 4.1 | 13 ± 4.13-152 | 20 ± 1.0 | 19 ± 3.4 | 4 ± 2.4 | 12 ± 5.83-152 |
| Activated | 28 ± 13 | 32 ± 10 | 21 ± 4.6 | 28 ± 6.83-152 | 37 ± 10 | 37 ± 8 | 22 ± 11.8 | 34 ± 8.03-152 |
| Memory | 50 ± 9.5 | 49 ± 12 | 60 ± 7.3 | 46 ± 7.63-150 | 27 ± 8.4 | 33 ± 1.8 | 61 ± 6.2 | 45 ± 8.03-151 |
Spleen cells from the indicated groups of mice (either BM alone or IL-tk transgenic T-cell recipients) treated with either PBS or GCV were recovered 4 weeks after treatment (6 weeks after transplantation) and analyzed by 4-color flow-activated cell sorter as described in “Materials and methods.” Cells were gated to analyze either CD4- (first 3 lines) or CD8- (last 3 lines) positive cells and then considered as naive (CD44lo/CD62hi), activated (CD44hi/CD62Lhi), or memory (CD44hi/CD62Llo) as described.44Shown are percentages ± SD. Experiment A used IL-2-tk donor T cells, and experiment B used IL-4-tk donor T cells. There was no difference among Tg− recipients treated with PBS or GCV, which were included in both experiments (not shown). Pvalues refer to comparisons between PBS- and GCV-treated groups receiving the same types of cells.
P < .001.
P < .01.
P < .05.