Effect of antileukemia CTL lines on the in vitro growth of clonogenic progenitor cells
| . | Patient 2 . | Patient 4 . | ||||
|---|---|---|---|---|---|---|
| BFU-E . | CFU-GM . | CFU-GEMM . | BFU-E . | CFU-GM . | CFU-GEMM . | |
| Experiment 1 | ||||||
| Control | 36 | 30 | 1 | 99 | 80 | 2 |
| PBL, 4 h | 38 | 28 | 1 | 81 | 68 | 1 |
| PBL, 7 h | 33 | 24 | 2 | 100 | 79 | 2 |
| CTL3, 4 h | 29 | 32 | 1 | ND | ND | ND |
| CTL3, 7 h | 30 | 24 | 1 | 86 | 70 | 1 |
| CTL4, 4 h | 33 | 25 | 0 | 79 | 71 | 2 |
| CTL4, 7 h | 29 | 25 | 1 | 79 | 74 | 1 |
| Experiment 2 | ||||||
| Control | 33 | 52 | 2 | 105 | 95 | 3 |
| CTL allo, 4 h | 10 | 17 | 0 | 29 | 19 | 0 |
| CTL allo, 7 h | 6 | 11 | 0 | 12 | 8 | 0 |
| . | Patient 2 . | Patient 4 . | ||||
|---|---|---|---|---|---|---|
| BFU-E . | CFU-GM . | CFU-GEMM . | BFU-E . | CFU-GM . | CFU-GEMM . | |
| Experiment 1 | ||||||
| Control | 36 | 30 | 1 | 99 | 80 | 2 |
| PBL, 4 h | 38 | 28 | 1 | 81 | 68 | 1 |
| PBL, 7 h | 33 | 24 | 2 | 100 | 79 | 2 |
| CTL3, 4 h | 29 | 32 | 1 | ND | ND | ND |
| CTL3, 7 h | 30 | 24 | 1 | 86 | 70 | 1 |
| CTL4, 4 h | 33 | 25 | 0 | 79 | 71 | 2 |
| CTL4, 7 h | 29 | 25 | 1 | 79 | 74 | 1 |
| Experiment 2 | ||||||
| Control | 33 | 52 | 2 | 105 | 95 | 3 |
| CTL allo, 4 h | 10 | 17 | 0 | 29 | 19 | 0 |
| CTL allo, 7 h | 6 | 11 | 0 | 12 | 8 | 0 |
LDBMCs were incubated in RPMI 1640 and 10% FCS in the presence of PBMCs or CTLs at a ratio of 1:3, respectively. After 4- and 7-hour incubations at 37°C, cells were washed and plated in methylcellulose in a classical clonogenic assay. Numbers represent the mean colony number of a clonogenic assay performed in duplicate.
CTL3 indicates CTL obtained after 3rd stimulation; CTL4, CTL obtained after 4th stimulation; CTL allo, CTL obtained after induction of a healthy volunteer's PBMCs with autologous DCs pulsed with irradiated patient 2 or patient 4 BMRCs, respectively; ND, not done.