Erythroid colony-forming units in fetal liver cells retrovirally transduced with BCR-ABL mutants
| BCR-ABL constructs . | Wild type (%) . | JAK2−/−(%) . |
|---|---|---|
| P210 | ND | 100(2) |
| P210 ΔSH2 | ND | 32 ± 4* |
| P210 Y177F | ND | 68 ± 7† |
| P185 | 100(1) | 399 ± 98† |
| P185Δ176-427 | 5.5 ± 2* | 15 ± 5* |
| P185 TM | 19.5 ± 4* | ND |
| BCR-ABL constructs . | Wild type (%) . | JAK2−/−(%) . |
|---|---|---|
| P210 | ND | 100(2) |
| P210 ΔSH2 | ND | 32 ± 4* |
| P210 Y177F | ND | 68 ± 7† |
| P185 | 100(1) | 399 ± 98† |
| P185Δ176-427 | 5.5 ± 2* | 15 ± 5* |
| P185 TM | 19.5 ± 4* | ND |
Results are obtained in the absence of Epo and are shown as a percentage of control ± se; the 100% control is determined by (1) expression of P185 in wild-type fetal liver resulting in generation of 5230 ± 1050 CFU-E per 105GFP+Ter119− FACS-sorted cells or (2) expression of P210 in JAK2−/− fetal liver resulting in generation of 1268 ± 221 per 105GFP+Ter119− FACS-sorted cells.
P < .001.
P < .01.
The P value for each mutant is determined by comparing numbers obtained for the mutant to the ones obtained for P185, in wild-type fetal liver cells, and to the numbers obtained for P210, in JAK2−/− fetal liver cells. ND indicates not determined.