Tetramer staining of T-cell lines generated against native and heteroclitic peptides
| Tetramer . | Healthy donors . | T cells generated against native peptide . | T cells generated against heteroclitic peptide . |
|---|---|---|---|
| Negative control | 0.04 | 0.30 | 0.06 |
| FR16 | 0.02 | 1.57 | 1.38 |
| FR16H | 0.06 | 2.34 | 9.96 |
| Negative control | 0.06 | 0.14 | 0.03 |
| FR17 | 0.04 | 2.07 | 2.61 |
| FR17H | 0.03 | 3.52 | 2.79 |
| Negative control | 0.02 | 0.04 | 0.04 |
| FR23 | 0.02 | 1.19 | 2.46 |
| FR23H | 0.02 | 1.95 | 2.13 |
| Tetramer . | Healthy donors . | T cells generated against native peptide . | T cells generated against heteroclitic peptide . |
|---|---|---|---|
| Negative control | 0.04 | 0.30 | 0.06 |
| FR16 | 0.02 | 1.57 | 1.38 |
| FR16H | 0.06 | 2.34 | 9.96 |
| Negative control | 0.06 | 0.14 | 0.03 |
| FR17 | 0.04 | 2.07 | 2.61 |
| FR17H | 0.03 | 3.52 | 2.79 |
| Negative control | 0.02 | 0.04 | 0.04 |
| FR23 | 0.02 | 1.19 | 2.46 |
| FR23H | 0.02 | 1.95 | 2.13 |
Shown is enumeration of peptide-specific T cells by tetramer staining of T cells from healthy donors and of T-cell lines generated against the framework region (FR) 16, FR17, and FR23 native and heteroclitic peptides. Shown is the frequency of CD8+ T cells generated against FR16, FR17, and FR23 and their heteroclitic counterparts that stain with specific tetramer, the heteroclitic tetramer, and a negative control tetramer.