Raised serum Epo in anemic Stat5a−/−5b−/− mice
| Mouse no. . | Genotype . | Hematocrit (%) . | Hemoglobin (g/dL) . | Serum Epo (mU/mL) . |
|---|---|---|---|---|
| 435 | 5a−/−5b−/− | 23 | 9.5 | 360 |
| 431 | 5a−/−5b−/− | 20 | 8.4 | 720 |
| 181 | 5a−/−5b−/− | 16 | ND | 4 000 |
| 436 | 5a−/−5b−/− | 4 | 1.0 | 12 000 |
| 573 | 5a−/−5b−/− | 36 | 11.9 | < 100 |
| 575 | 5a−/−5b−/− | 45 | 13.6 | < 100 |
| 449 | Wild-type | 45 | 17.9 | < 100 |
| 557 | Wild-type | 46 | 15 | < 100 |
| Mouse no. . | Genotype . | Hematocrit (%) . | Hemoglobin (g/dL) . | Serum Epo (mU/mL) . |
|---|---|---|---|---|
| 435 | 5a−/−5b−/− | 23 | 9.5 | 360 |
| 431 | 5a−/−5b−/− | 20 | 8.4 | 720 |
| 181 | 5a−/−5b−/− | 16 | ND | 4 000 |
| 436 | 5a−/−5b−/− | 4 | 1.0 | 12 000 |
| 573 | 5a−/−5b−/− | 36 | 11.9 | < 100 |
| 575 | 5a−/−5b−/− | 45 | 13.6 | < 100 |
| 449 | Wild-type | 45 | 17.9 | < 100 |
| 557 | Wild-type | 46 | 15 | < 100 |
Epo was measured using a sandwich ELISA in several anemic and nonanemic Stat5a−/−5b−/− mice and in wild-type littermate controls. Serum Epo below 100 mU/mL could not be reliably measured by this assay. Normal mouse serum Epo is in the range of 10 to 20 mU/mL.22
ND indicates not done.