Hematologic parameters of Stat5a−/−5b−/− mice
| . | Age . | Genotype (no. of animals) . | Mean ± SEM . | P . |
|---|---|---|---|---|
| Hematocrit (%) | Neonates | 5a−/−5b−/−(65) | 28.9 ± 0.9*,† | *P< .0001 †P < .0001 |
| 5a+/−5b+/−(88) | 41.3 ± 0.5* | *P = .0014 | ||
| Wild-type (12) | 47.6 ± 1.6 | |||
| 3 wk | 5a−/−5b−/−(12) | 32.6 ± 1.0*,† | *P< .0001 †P = .03 | |
| 5a+/−5b+/−(23) | 36.5 ± 1.2* | *P = .0006 | ||
| Wild-type (5) | 45.7 ± 1.2 | |||
| 5-12 wk | 5a−/−5b−/−(26) | 27.9 ± 2.0* | *P < .0001 | |
| 5a+/−5b+/−(3) | 29.5 ± 6.2* | *P = .008 | ||
| Wild-type (15) | 45.2 ± 0.9 | |||
| 3-6 mo | 5a−/−5b−/−(21) | 41.1 ± 1.9* | *P = .0014 | |
| 5a+/−5b+/−(5) | 44.0 ± 2.6 | |||
| Wild-type (15) | 49.2 ± 1.0 | |||
| MCV (μ3) | Neonates | 5a−/−5b−/−(14) | 89.7 ± 1.6*,† | *P< .0001 †P = .0003 |
| 5a+/−5b+/−(25) | 97.3 ± 1.25 | |||
| Wild-type (9) | 102.8 ± 1.5 | |||
| 3 wk | 5a−/−5b−/−(10) | 56.1 ± 0.73*,† | *P= .004 †P = .002 | |
| 5a+/−5b+/−(18) | 59.1 ± 0.45 | |||
| Wild-type (5) | 60.0 ± 1.47 | |||
| 5 wk | 5a−/−5b−/−(10) | 51.8 ± 2.75 | ||
| 5a+/−5b+/−(18) | 54.6 ± 1.1 | |||
| Wild-type (5) | 54.6 ± 1.3 | |||
| Total bilirubin (mg/dL) | >6 wk | 5a−/−5b−/−(9) | 0.46 ± 0.19 | |
| Wild-type (6) | 0.12 ± 0.02 | |||
| LDH (IU/L) | 5a−/−5b−/−(9) | 715 ± 372 | ||
| Wild-type (6) | 282 ± 120 | |||
| Serum iron (μg/dL) | 5a−/−5b−/−(6) | 161.3 ± 15.1 | ||
| Wild-type (6) | 152.7 ± 17.9 | |||
| TIBC (μg/dL) | 5a−/−5b−/−(6) | 617 ± 40.0* | *P < .0001 | |
| Wild-type (6) | 427 ± 18.8 |
| . | Age . | Genotype (no. of animals) . | Mean ± SEM . | P . |
|---|---|---|---|---|
| Hematocrit (%) | Neonates | 5a−/−5b−/−(65) | 28.9 ± 0.9*,† | *P< .0001 †P < .0001 |
| 5a+/−5b+/−(88) | 41.3 ± 0.5* | *P = .0014 | ||
| Wild-type (12) | 47.6 ± 1.6 | |||
| 3 wk | 5a−/−5b−/−(12) | 32.6 ± 1.0*,† | *P< .0001 †P = .03 | |
| 5a+/−5b+/−(23) | 36.5 ± 1.2* | *P = .0006 | ||
| Wild-type (5) | 45.7 ± 1.2 | |||
| 5-12 wk | 5a−/−5b−/−(26) | 27.9 ± 2.0* | *P < .0001 | |
| 5a+/−5b+/−(3) | 29.5 ± 6.2* | *P = .008 | ||
| Wild-type (15) | 45.2 ± 0.9 | |||
| 3-6 mo | 5a−/−5b−/−(21) | 41.1 ± 1.9* | *P = .0014 | |
| 5a+/−5b+/−(5) | 44.0 ± 2.6 | |||
| Wild-type (15) | 49.2 ± 1.0 | |||
| MCV (μ3) | Neonates | 5a−/−5b−/−(14) | 89.7 ± 1.6*,† | *P< .0001 †P = .0003 |
| 5a+/−5b+/−(25) | 97.3 ± 1.25 | |||
| Wild-type (9) | 102.8 ± 1.5 | |||
| 3 wk | 5a−/−5b−/−(10) | 56.1 ± 0.73*,† | *P= .004 †P = .002 | |
| 5a+/−5b+/−(18) | 59.1 ± 0.45 | |||
| Wild-type (5) | 60.0 ± 1.47 | |||
| 5 wk | 5a−/−5b−/−(10) | 51.8 ± 2.75 | ||
| 5a+/−5b+/−(18) | 54.6 ± 1.1 | |||
| Wild-type (5) | 54.6 ± 1.3 | |||
| Total bilirubin (mg/dL) | >6 wk | 5a−/−5b−/−(9) | 0.46 ± 0.19 | |
| Wild-type (6) | 0.12 ± 0.02 | |||
| LDH (IU/L) | 5a−/−5b−/−(9) | 715 ± 372 | ||
| Wild-type (6) | 282 ± 120 | |||
| Serum iron (μg/dL) | 5a−/−5b−/−(6) | 161.3 ± 15.1 | ||
| Wild-type (6) | 152.7 ± 17.9 | |||
| TIBC (μg/dL) | 5a−/−5b−/−(6) | 617 ± 40.0* | *P < .0001 | |
| Wild-type (6) | 427 ± 18.8 |
All measurements were carried out on Stat5a−/−5b−/− mice of the ages indicated, and on Stat5a−/−5b−/−, Stat5a+/−5b+/− or wild-type controls from the same mouse colony. Hematocrit data corresponds to Figure 1.P values are indicated only when P < .05. The increase in total iron-binding capacity (TIBC) in Stat5a−/−5b−/− mice is consistent with ineffective erythropoiesis. Red cell indices in Stat5a−/−5b−/− mice other than MCV were not significantly different than control values. P was calculated using Fisher protected least significant difference (PLSD) post hoc analysis.
Significantly different from wild-type value.
Significantly different from Stat5a+/−5b+/− value.