Analysis of expression of clones from stable cell lines
| Cell type . | Construct . | Clones expressing the reporter/total clones with transgene . |
|---|---|---|
| U937 human myeloid (PU.1+) | Dnase I HS + PU.1 promoter | 14/14 |
| U937 human myeloid (PU.1+) | PU.1 promoter only | 10/14 |
| 416B murine myeloid (PU.1+) | Dnase I HS + PU.1 promoter | 18/18 |
| 416B murine myeloid (PU.1+) | PU.1 promoter only | 14/16 |
| BW5147 murine T cell (PU.1−) | Dnase I HS + PU.1 promoter | 18/18 |
| BW5147 murine T cell (PU.1−) | PU.1 promoter only | 15/18 |
| Cell type . | Construct . | Clones expressing the reporter/total clones with transgene . |
|---|---|---|
| U937 human myeloid (PU.1+) | Dnase I HS + PU.1 promoter | 14/14 |
| U937 human myeloid (PU.1+) | PU.1 promoter only | 10/14 |
| 416B murine myeloid (PU.1+) | Dnase I HS + PU.1 promoter | 18/18 |
| 416B murine myeloid (PU.1+) | PU.1 promoter only | 14/16 |
| BW5147 murine T cell (PU.1−) | Dnase I HS + PU.1 promoter | 18/18 |
| BW5147 murine T cell (PU.1−) | PU.1 promoter only | 15/18 |
Clones were considered to be expressing the reporter if luciferase activity was 3-fold higher than background. The luciferase activity of the average of expressing clones is shown in Figure4B.