Kinetic and binding properties of TPIs from blood cells
| . | TPI activity expected, % . | TPI activity measured in hemolysates U/g hemoglobin . | TPI activity measured in lysates of platelets U/1010 cells . | DHAP level in RBC μM RBCs . | DHAP level in platelets nmol/1010 cells . | Binding from hemolysates . | Binding from platelet lysates . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| to IOV . | to MT . | to MT . | ||||||||
| −PEG, % . | +PEG, % . | −PEG, % . | +PEG, % . | −PEG, % . | ||||||
| Control | 100 | 1398 ± 361 | 181 | 11.4 ± 0.7 | 136 | 0.53 | 1.1 | 1.5 | 3.1 | 1.4 |
| Mother | 65 | 553 ± 84 | 120.7 | 19.8 | 188 | 1.3 | 2.7 | 5.9 | 7.4 | 1.9 |
| Father | 50 | 772 ± 123 | 86.8 | 23.5 | 183 | n.m. | n.m. | 2.2 | 10.8 | 1.3 |
| Brother | 15 | 40.9 ± 17.9 | 39.8 | 547 ± 53 | 163 | 6.5 | 10.2 | 5.0 | 12.0 | 6.9 |
| Propositus | 15 | 33.0 ± 16.4 | 39.7 | 701 ± 98 | 267 | 4.2 | 5.3 | 11.0 | 11.0 | 4.3 |
| British | — | 29.8 | n.m. | 935 | n.m. | 4.9 | 5.9 | 18.8 | 18.7 | n.m. |
| . | TPI activity expected, % . | TPI activity measured in hemolysates U/g hemoglobin . | TPI activity measured in lysates of platelets U/1010 cells . | DHAP level in RBC μM RBCs . | DHAP level in platelets nmol/1010 cells . | Binding from hemolysates . | Binding from platelet lysates . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| to IOV . | to MT . | to MT . | ||||||||
| −PEG, % . | +PEG, % . | −PEG, % . | +PEG, % . | −PEG, % . | ||||||
| Control | 100 | 1398 ± 361 | 181 | 11.4 ± 0.7 | 136 | 0.53 | 1.1 | 1.5 | 3.1 | 1.4 |
| Mother | 65 | 553 ± 84 | 120.7 | 19.8 | 188 | 1.3 | 2.7 | 5.9 | 7.4 | 1.9 |
| Father | 50 | 772 ± 123 | 86.8 | 23.5 | 183 | n.m. | n.m. | 2.2 | 10.8 | 1.3 |
| Brother | 15 | 40.9 ± 17.9 | 39.8 | 547 ± 53 | 163 | 6.5 | 10.2 | 5.0 | 12.0 | 6.9 |
| Propositus | 15 | 33.0 ± 16.4 | 39.7 | 701 ± 98 | 267 | 4.2 | 5.3 | 11.0 | 11.0 | 4.3 |
| British | — | 29.8 | n.m. | 935 | n.m. | 4.9 | 5.9 | 18.8 | 18.7 | n.m. |
The data are the mean of 3 to 10 measurements. SEs are given only if at least 5 different sets of experiments were carried out.
n.m. indicates not measured.