Table 1. Short-term engraftment using... | American Society of Hematology

Table 1.

Short-term engraftment using normal (+/+) or CD18^−/− donor cells

Donor cells	Donors			Recipients
	No. BM cells × 10³ injected	No. CFU-Cs injected	n	Femur		PB			Spleen		Femur CFU-C amplification^*
	No. BM cells × 10³ injected	No. CFU-Cs injected	n	Cells × 10³	CFU-C	WBC	Hct	CD18+	CFU-S	GR-1+	Femur CFU-C amplification^*
8 days after transplantation^†
+/+	1 500	3 940	5	1 970 ± 232	965 ± 271						49×
−/−^*	1 500	3 520	5	1 930 ± 227	212 ± 113						12×^1-153
+/+	6 760	10 440	5	9 890 ± 1 231	6 241 ± 1 108						120×
−/−	6 720	13 860	5	14 100 ± 928	9 236 ± 387						133×
14 days after transplantation^‡
+/+	500	1 240	5	8 863 ± 629	4 327 ± 2 100	5 077 ± 379	42.1% ± 1.4		6.5		698×
−/−	500	1 395	6	9 230 ± 1 033	4 627 ± 1 155	7 544 ± 897	43.9% ± 0.5		6.8		663×
43 days after transplantation
+/+	100	4 327	7			7 713 ± 822		54.2% ± 6		19.3% ± 5
−/−	100	4 627	6			14 760 ± 2 520		19.1 ± 4		22.3% ± 5

Donor cells	Donors			Recipients
	No. BM cells × 10³ injected	No. CFU-Cs injected	n	Femur		PB			Spleen		Femur CFU-C amplification^*
	No. BM cells × 10³ injected	No. CFU-Cs injected	n	Cells × 10³	CFU-C	WBC	Hct	CD18+	CFU-S	GR-1+	Femur CFU-C amplification^*
8 days after transplantation^†
+/+	1 500	3 940	5	1 970 ± 232	965 ± 271						49×
−/−^*	1 500	3 520	5	1 930 ± 227	212 ± 113						12×^1-153
+/+	6 760	10 440	5	9 890 ± 1 231	6 241 ± 1 108						120×
−/−	6 720	13 860	5	14 100 ± 928	9 236 ± 387						133×
14 days after transplantation^‡
+/+	500	1 240	5	8 863 ± 629	4 327 ± 2 100	5 077 ± 379	42.1% ± 1.4		6.5		698×
−/−	500	1 395	6	9 230 ± 1 033	4 627 ± 1 155	7 544 ± 897	43.9% ± 0.5		6.8		663×
43 days after transplantation
+/+	100	4 327	7			7 713 ± 822		54.2% ± 6		19.3% ± 5
−/−	100	4 627	6			14 760 ± 2 520		19.1 ± 4		22.3% ± 5

WBC indicates white blood cell; hct, hematocrit; and PB, peripheral blood.

Amplification factor was calculated by comparing the initial (at 3 hours) value of CFU-Cs per femur (based on homing of 0.5% of injected CFU-Cs) and the CFU-C/femur measured at 8 or 14 days later.

†

Two independent experiments are shown and none of the donor animals were pretreated with antibiotics. Each group had 5 mice.

‡

Recipients of 5 × 10⁵ BM cells were sacrificed at 14 days, whereas recipients of 1 × 10⁵BM cells (from the same BM pool) were bled at 43 days after transplant to assess engraftment.

F1-153

P = .0674.

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