Table 2.

Cell cycle analysis performed by flow cytometry after 24 hours of treatment with SNP, TRAIL, and SNP plus TRAIL

Cells and treatmentPercentage of cells
G1SG2/M
HEL    
 Control 41 31 28 
 SNP 33 39 28  
 TRAIL 36 37 26  
 TRAIL + SNP 40 40 20 
HL-60    
 Control 66 21 13 
 SNP 45 36 19  
 TRAIL 50 39 11  
 TRAIL + SNP 56 32 12 
Jurkat    
 Control 63 27 10 
 SNP 50 32 18  
 TRAIL 54 33 13  
 TRAIL + SNP 50 41 
SupT1    
 Control 73 16 11 
 SNP 60 23 17  
 TRAIL 54 33 13  
 TRAIL + SNP 58 33 9  
Normal erythroblast    
 Control 38 41 21 
 SNP 30 50 20  
 TRAIL 37 43 20  
 TRAIL + SNP 33 48 19  
Normal granulocytic cells    
 Control 73 21 
 SNP 72 22 6  
 TRAIL 72 23 5  
 TRAIL + SNP 71 23 
Cells and treatmentPercentage of cells
G1SG2/M
HEL    
 Control 41 31 28 
 SNP 33 39 28  
 TRAIL 36 37 26  
 TRAIL + SNP 40 40 20 
HL-60    
 Control 66 21 13 
 SNP 45 36 19  
 TRAIL 50 39 11  
 TRAIL + SNP 56 32 12 
Jurkat    
 Control 63 27 10 
 SNP 50 32 18  
 TRAIL 54 33 13  
 TRAIL + SNP 50 41 
SupT1    
 Control 73 16 11 
 SNP 60 23 17  
 TRAIL 54 33 13  
 TRAIL + SNP 58 33 9  
Normal erythroblast    
 Control 38 41 21 
 SNP 30 50 20  
 TRAIL 37 43 20  
 TRAIL + SNP 33 48 19  
Normal granulocytic cells    
 Control 73 21 
 SNP 72 22 6  
 TRAIL 72 23 5  
 TRAIL + SNP 71 23 

The percentage of cells with a G1(2n), S, G2/M(4n) DNA content, were calculated after removal of the apoptotic cells. Data represent the means of 3 separate experiments. SDs were comprised within 10% of the means and are omitted for clarity.

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