Frequency of TRAP-positive cells (M-CSF and sRANKL) and DEC205-positive cells (GM-CSF and sRANKL) developed from single common progenitors
| . | Experiment 1 (%) . | Experiment 2 (%) . |
|---|---|---|
| M-CSF and sRANKL | 56/96 (58.3) | 38/96 (39.6) |
| GM-CSF and sRANKL | 48/88 (54.5) | 32/96 (33.3) |
| . | Experiment 1 (%) . | Experiment 2 (%) . |
|---|---|---|
| M-CSF and sRANKL | 56/96 (58.3) | 38/96 (39.6) |
| GM-CSF and sRANKL | 48/88 (54.5) | 32/96 (33.3) |
The c-Kit+c-Fms+RANK− cells were clone-sorted and cultured in the presence of M-CSF/GM-CSF and sRANKL for 6 days and stained with TRAP and DEC205, respectively. The number of wells containing TRAP-positive and DEC205-positive cells was scored.