Table 1. Response to interferons and... | American Society of Hematology

Table 1.

Response to interferons and Apo2L of plasma cells isolated from the bone marrow of MM patients

Patient no.	Age^* (y)	% of PCs at Dx^†	Previous treatment	% of PCs at selection^†	% of PCs treated^‡	Apoptosis (%)
Patient no.	Age^* (y)	% of PCs at Dx^†	Previous treatment	% of PCs at selection^†	% of PCs treated^‡	Control	α	β	γ	Apo2L
1	66	80	CTX × 6	Clusters	90	8.7	15	15	ND	19
2	77	100	RTX × 4	90	79	3.2	26	22	8	53
3	69	80	RTX × 4	51	80	9.4	38	23	ND	31
4	74	70	RTX × 4	80	81	3.2	24	13	ND	15
5	46	80	No	80	100	5.2	31	27	5	31
6	45	8	RMP, DVD × 8, CTX × 4	ND	90	5.2	25	21	14	85
7	51	30	RTX × 4	20	90	6.3	36	34	17	86
8B	44	60-70	VAD × 3	75	9	1.9	2	13	4	11
			CTX × 2
			DVD, RTX, De, DT
8C					90	6.5	23	32	26	27
9B	61	55	No	55	0	2.7	2	4	1	5
9C					85	2.7	21	28	13	49
10B	59	50	No	10-20	6	1.5	8	6	6	3
10C					86	5.3	23	16	20	15
5B^1-153	46	80	RTX × 2	ND	26	3.6	ND	7	10	5
5C^1-153					92	6.7	ND	33	5	31

Patient no.	Age^* (y)	% of PCs at Dx^†	Previous treatment	% of PCs at selection^†	% of PCs treated^‡	Apoptosis (%)
Patient no.	Age^* (y)	% of PCs at Dx^†	Previous treatment	% of PCs at selection^†	% of PCs treated^‡	Control	α	β	γ	Apo2L
1	66	80	CTX × 6	Clusters	90	8.7	15	15	ND	19
2	77	100	RTX × 4	90	79	3.2	26	22	8	53
3	69	80	RTX × 4	51	80	9.4	38	23	ND	31
4	74	70	RTX × 4	80	81	3.2	24	13	ND	15
5	46	80	No	80	100	5.2	31	27	5	31
6	45	8	RMP, DVD × 8, CTX × 4	ND	90	5.2	25	21	14	85
7	51	30	RTX × 4	20	90	6.3	36	34	17	86
8B	44	60-70	VAD × 3	75	9	1.9	2	13	4	11
			CTX × 2
			DVD, RTX, De, DT
8C					90	6.5	23	32	26	27
9B	61	55	No	55	0	2.7	2	4	1	5
9C					85	2.7	21	28	13	49
10B	59	50	No	10-20	6	1.5	8	6	6	3
10C					86	5.3	23	16	20	15
5B^1-153	46	80	RTX × 2	ND	26	3.6	ND	7	10	5
5C^1-153					92	6.7	ND	33	5	31

PC, plasma cells; Dx, diagnosis; CTX, Cytoxan; ND, not detected; RTX, Rituxan; RMP, ritoxin + melphalan + prednisone; DVD, doxorubicin + vincristine + dexamethasone; B, C, nonplasma and plasma cells after CD138 selection; VAD, vincristine + adriamycin + doxorubicin; De, Decadron; DT, DTPASE.

PCs were isolated from the bone marrow of patients with MM by negative (patients 1-4) or positive selection. The plasma B cells (enriched more than 80%) were treated with IFN-α, β, or γ (2000 U/mL) or with Apo2L (400 ng/mL) for 48 hours. The apoptotic cells (> 200/sample) were then stained with Hoechst 33258, scored by fluorescence microscopy, and expressed as the percentage of the total cells.

Age at first diagnosis of multiple myeloma.

†

Identified from medical files and proved by pathologist.

‡

Percentage of PCs in the samples treated with interferon (α, β, or γ) or with Apo2L.

F1-153

This patient was seen twice: at diagnosis and 2 months later.

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