Primer and probe sequences
| ORP-3 RACE . | |
|---|---|
| Nested reverse race primer 1 | 5′-TGA GCA GAC GGG CAG GAG GAG G-3′ |
| Nested reverse race primer 2 | 5′-TGG ACC GCC ACC TCC TGT GC-3′ |
| Nested reverse race primer 3 | 5′-AGG CAT GAC AGT GCG CCA GGT C-3′ |
| Nested reverse race primer 4 | 5′-TCT GAC GAT ACA TTC TGT GGT GGC GAA G-3′ |
| Nested reverse race primer 5 | 5′-TCA TCT CCC CCC TCA GTC CTT CCA CC-3′ |
| Nested forward race primer 1 | 5′-CCA AAG CAC CCC AAG GCT GGT ATT C-3′ |
| Nested forward race primer 2 | 5′-GTC CTG TGC TTC CTG TGA GAC TTC CTG G-3′ |
| Nested forward race primer 3 | 5′-GCT TCA AGC ACT GTC TTC CAT CTG CAT C-3′ |
| Northern hybridization | |
| ORP-3 forward | 5′-GCT CAG GAA GTT CTG TTA TCT CC-3′ |
| ORP-3 reverse | 5′-GCT AAG CTA AGC ACA AGT GAT CA-3′ |
| Real-time | |
| ORP-3 forward | 5′-CAA ACT GGA CCA TCC TGT CTT ATG-3′ |
| ORP-3 reverse | 5′-AAG CTA AGC ACA AGT GAT CAT CCT AGA-3′ |
| ORP-3 probe | fam-AAC ATT AGT GTA TTT CTC CTG TGC TTG C-tamra |
| β-actin forward | 5′-GAC AGG ATG CAG AAG GAG ATT ACT-3′ |
| β-actin reverse | 5′-TGA TCC ACA TCT GCT GGA AGG T-3′ |
| β-actin probe | fam-ATC ATT GCT CCT CCT GAG CGC AAG TAC TC-tamra |
| ORP-3 RACE . | |
|---|---|
| Nested reverse race primer 1 | 5′-TGA GCA GAC GGG CAG GAG GAG G-3′ |
| Nested reverse race primer 2 | 5′-TGG ACC GCC ACC TCC TGT GC-3′ |
| Nested reverse race primer 3 | 5′-AGG CAT GAC AGT GCG CCA GGT C-3′ |
| Nested reverse race primer 4 | 5′-TCT GAC GAT ACA TTC TGT GGT GGC GAA G-3′ |
| Nested reverse race primer 5 | 5′-TCA TCT CCC CCC TCA GTC CTT CCA CC-3′ |
| Nested forward race primer 1 | 5′-CCA AAG CAC CCC AAG GCT GGT ATT C-3′ |
| Nested forward race primer 2 | 5′-GTC CTG TGC TTC CTG TGA GAC TTC CTG G-3′ |
| Nested forward race primer 3 | 5′-GCT TCA AGC ACT GTC TTC CAT CTG CAT C-3′ |
| Northern hybridization | |
| ORP-3 forward | 5′-GCT CAG GAA GTT CTG TTA TCT CC-3′ |
| ORP-3 reverse | 5′-GCT AAG CTA AGC ACA AGT GAT CA-3′ |
| Real-time | |
| ORP-3 forward | 5′-CAA ACT GGA CCA TCC TGT CTT ATG-3′ |
| ORP-3 reverse | 5′-AAG CTA AGC ACA AGT GAT CAT CCT AGA-3′ |
| ORP-3 probe | fam-AAC ATT AGT GTA TTT CTC CTG TGC TTG C-tamra |
| β-actin forward | 5′-GAC AGG ATG CAG AAG GAG ATT ACT-3′ |
| β-actin reverse | 5′-TGA TCC ACA TCT GCT GGA AGG T-3′ |
| β-actin probe | fam-ATC ATT GCT CCT CCT GAG CGC AAG TAC TC-tamra |