Secondary reconstitution potential of different murine hematopoietic cell types
| Primary cells transplanted . | Percent donor (no. secondary mice) . | Percent donor cells among B cells . | Percent donor cells among T cells . | Percent donor cells among myeloid cells . | CFC/femur . |
|---|---|---|---|---|---|
| 2 × 106BM | 68 ± 3 (11) | 90 ± 2 | 22 ± 1 | 85 ± 2 | 35 820 ± 6 120 |
| 2 × 106FL | 92 ± 1 (12) | 99 ± 1 | 75 ± 2 | 93 ± 1 | 37 860 ± 3 390 |
| 2 × 106MPB | 51 ± 10 (12) | 64 ± 11 | 38 ± 7 | 48 ± 11 | 38 540 ± 2 800 |
| CFC normalized FL | 86 ± 1 (9) | 100 ± 0 | 40 ± 1 | 95 ± 1 | 43 390 ± 3 110 |
| CFC normalized MPB | 56 ± 15 (3) | 71 ± 18 | 47 ± 14 | 36 ± 19 | 42 830 ± 2 730 |
| Primary cells transplanted . | Percent donor (no. secondary mice) . | Percent donor cells among B cells . | Percent donor cells among T cells . | Percent donor cells among myeloid cells . | CFC/femur . |
|---|---|---|---|---|---|
| 2 × 106BM | 68 ± 3 (11) | 90 ± 2 | 22 ± 1 | 85 ± 2 | 35 820 ± 6 120 |
| 2 × 106FL | 92 ± 1 (12) | 99 ± 1 | 75 ± 2 | 93 ± 1 | 37 860 ± 3 390 |
| 2 × 106MPB | 51 ± 10 (12) | 64 ± 11 | 38 ± 7 | 48 ± 11 | 38 540 ± 2 800 |
| CFC normalized FL | 86 ± 1 (9) | 100 ± 0 | 40 ± 1 | 95 ± 1 | 43 390 ± 3 110 |
| CFC normalized MPB | 56 ± 15 (3) | 71 ± 18 | 47 ± 14 | 36 ± 19 | 42 830 ± 2 730 |
BM cells from primary recipients described in Table 1 were retransplanted into secondary lethally irradiated Ly-5.1 mice (0.5 femur equivalent/mouse). Ten weeks later, the percent of donor-derived cells in various lineages of peripheral blood cells was determined by flow cytometry. Hematopoietic progenitors in the BM were assayed by plating in semisolid medium. All values are expressed as the mean ± SEM.