Membrane-tethered or cytoplasmic Mpl can support the generation of progenitor cells
| Cellular compartment . | Colonies/105 cells plated . | ||
|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Meg . | |
| Mpl (membrane) | 2590 | 173 ± 17 | 10 ± 3 |
| Mpl (cytoplasmic) | 1867 | 105 ± 10 | 5 ± 1 |
| Cellular compartment . | Colonies/105 cells plated . | ||
|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Meg . | |
| Mpl (membrane) | 2590 | 173 ± 17 | 10 ± 3 |
| Mpl (cytoplasmic) | 1867 | 105 ± 10 | 5 ± 1 |
Marrow cells transduced with either membrane-targeted Mpl or cytoplasmic Mpl were cultured in suspension in the presence of AP20187 (100 nM), and on day 33 progenitor cell assays were performed. Data show the average of duplicate plates (CFU-GM) or the mean ± SEM of triplicate plates (BFU-E, CFU-Meg). Colony assays from a second independent transduction of marrow cells produced similar results.