Detection of tumor-reactive antibodies in the A20 lymphoma model
| Group . | Mice . | % reactivity with A20 cells (preimmune sera) . | % reactivity with A20 cells (sera after vaccination on day 40) . | Vaccination treatment . |
|---|---|---|---|---|
| A | 1 | 1 | 40 | Application of intact bsAb BiLu and irradiated tumor cells |
| 2 | 1 | 46 | ||
| 3 | 1 | 18 | ||
| 4 | 1 | 29 | ||
| 5 | 2 | 28 | ||
| 6 | 1 | 30 | ||
| B | 7 | 2 | 4 | Application of intact bsAb BiLu and irradiated tumor cells; depletion of CD4+ T cells |
| 8 | 1 | 14 | ||
| 9 | 2 | 9 | ||
| 10 | 2 | 5 | ||
| 11 | 2 | 3 | ||
| 12 | 2 | 15 | ||
| C | 13 | 1 | 1 | Application of bsF(ab′)2 fragments and irradiated tumor cells |
| 14 | 2 | 1 | ||
| 15 | 2 | 1 | ||
| 16 | 2 | 1 | ||
| 17 | 2 | 2 | ||
| 18 | 1 | 2 | ||
| D | 19 | 1 | 2 | Control: application of irradiated tumor cells without antibody |
| 20 | 1 | 3 | ||
| 21 | 2 | 4 | ||
| 22 | 1 | 1 | ||
| 23 | 1 | 1 | ||
| 24 | 1 | 1 |
| Group . | Mice . | % reactivity with A20 cells (preimmune sera) . | % reactivity with A20 cells (sera after vaccination on day 40) . | Vaccination treatment . |
|---|---|---|---|---|
| A | 1 | 1 | 40 | Application of intact bsAb BiLu and irradiated tumor cells |
| 2 | 1 | 46 | ||
| 3 | 1 | 18 | ||
| 4 | 1 | 29 | ||
| 5 | 2 | 28 | ||
| 6 | 1 | 30 | ||
| B | 7 | 2 | 4 | Application of intact bsAb BiLu and irradiated tumor cells; depletion of CD4+ T cells |
| 8 | 1 | 14 | ||
| 9 | 2 | 9 | ||
| 10 | 2 | 5 | ||
| 11 | 2 | 3 | ||
| 12 | 2 | 15 | ||
| C | 13 | 1 | 1 | Application of bsF(ab′)2 fragments and irradiated tumor cells |
| 14 | 2 | 1 | ||
| 15 | 2 | 1 | ||
| 16 | 2 | 1 | ||
| 17 | 2 | 2 | ||
| 18 | 1 | 2 | ||
| D | 19 | 1 | 2 | Control: application of irradiated tumor cells without antibody |
| 20 | 1 | 3 | ||
| 21 | 2 | 4 | ||
| 22 | 1 | 1 | ||
| 23 | 1 | 1 | ||
| 24 | 1 | 1 |
bsAb indicates bispecific antibody.
Sera of immunized BALB/c mice were assessed for tumor-reactive antibodies by flow-activated cell sorter analysis as described in “Materials and methods.” The reaction is shown as the percentage of positively stained tumor cells. P < .0023 for the differences between group A and the other groups B-D, by the Mann-Whitney U test.