Frequency of clonally derived primary and secondary neurospheres from fetal brains that differentiate into multilineage brain cells in vitro
| Donor no. . | EGF-generated neurospheres . | FGF-2–generated neurospheres . | (EGF + FGF-2)–generated neurospheres . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phenotype composition . | Phenotype composition . | Phenotype composition . | ||||||||||
| Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | |||||||
| N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | |
| 1 | 80 | 13 | 91 | 4 | 67 | 22 | 78 | 14 | 88 | 9 | 91 | 6 |
| 2 | 85 | 9 | 91 | 4 | 70 | 20 | 80 | 16 | 87 | 7 | 90 | 5 |
| 3 | 82 | 11 | 93 | 5 | 68 | 20 | 76 | 15 | 88 | 9 | 91 | 5 |
| Donor no. . | EGF-generated neurospheres . | FGF-2–generated neurospheres . | (EGF + FGF-2)–generated neurospheres . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phenotype composition . | Phenotype composition . | Phenotype composition . | ||||||||||
| Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | Primary neurospheres . | Secondary neurospheres . | |||||||
| N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | N + A + O . | N + G . | |
| 1 | 80 | 13 | 91 | 4 | 67 | 22 | 78 | 14 | 88 | 9 | 91 | 6 |
| 2 | 85 | 9 | 91 | 4 | 70 | 20 | 80 | 16 | 87 | 7 | 90 | 5 |
| 3 | 82 | 11 | 93 | 5 | 68 | 20 | 76 | 15 | 88 | 9 | 91 | 5 |
Individual spheres were transferred onto poly-L-ornithine–coated glass coverslips and cultured in differentiation medium for 8 to 10 days before processing for triple-labeled immunocytochemistry. The number of differentiated spheres containing all 3 lineages (N + A + O) or neurons and either astrocytes or oligodendrocytes (N + G) are presented. The data presented are the actual number in each category of a total of 100 spheres derived from each culture condition. These data are compiled from 3 independent experiments using different donor brain samples. EGF indicates epidermal growth factor; FGF-2, fibroblast growth factor; N, neurons; A, astrocytes; and O, oligodentrocytes.