MV-Edm infection induced extensive syncytial formation in ARH-77 myeloma cell line and primary myeloma cells but not in PHA-activated PBLs
| Cells . | Percentage of infected cells/syncytia (size of syncytia) . | ||
|---|---|---|---|
| MOI 0.1 . | MOI 1.0 . | MOI 10.0 . | |
| ARH-77 | 50% (+++) | > 90% (+++) | > 90% (+++) |
| Patient 1 | 10% (+++) | 60% (+++) | > 90% (++) |
| Patient 2 | 10% (+++) | 60% (++) | 80% (++) |
| Patient 3 | ND | 40% (+++) | 70% (+) |
| Patient 4 | ND | 30% (++) | 60% (+) |
| Patient 5 | ND | 50% (+++) | 80% (+++) |
| Patient 6 | 30% (+++) | 50% (+++) | 30% (+++) |
| PBLs | ND | 24% (±) | 11% (±)* |
| Cells . | Percentage of infected cells/syncytia (size of syncytia) . | ||
|---|---|---|---|
| MOI 0.1 . | MOI 1.0 . | MOI 10.0 . | |
| ARH-77 | 50% (+++) | > 90% (+++) | > 90% (+++) |
| Patient 1 | 10% (+++) | 60% (+++) | > 90% (++) |
| Patient 2 | 10% (+++) | 60% (++) | 80% (++) |
| Patient 3 | ND | 40% (+++) | 70% (+) |
| Patient 4 | ND | 30% (++) | 60% (+) |
| Patient 5 | ND | 50% (+++) | 80% (+++) |
| Patient 6 | 30% (+++) | 50% (+++) | 30% (+++) |
| PBLs | ND | 24% (±) | 11% (±)* |
The percentage of fluorescent cells/syncytia was noted 3 to 4 days after infection by MV-eGFP. Average syncytial size was scored by estimating the average number of cells per syncytium.
+++ indicates more than 50 cells per syncytium; ++, 21-50 cells per syncytium; +, 6-20 cells per syncytium; ±, 5 or fewer cells per syncytium; ND, not done.
MOI = 100.