Sequences of cloned TCRD recombination products
| Sample . | 5′ gene segment* . | P nucleotides . | 3′ gene segment* . |
|---|---|---|---|
| Vδ2-Dδ3 rearrangements | |||
| Germline† | TGT GCC TGT GAC ACC | ACT GGG GGA TAC G cac agt g | |
| E47/RR transfectant | 0 | −3 | |
| 0 | −4 | ||
| HEB/RR transfectant | −2 | −3 | |
| −1 | −2 | ||
| −1 | −3 | ||
| −2 | T | 0 | |
| 0 | −3 | ||
| Dδ2-Dδ3 rearrangements‡ | |||
| Germline2-153 | c att gtg CCT TCC TAC | ACT GGG GGA TAC G cac agt g | |
| E12/RR transfectant (4/4)# | c att gtg | cac agt g | |
| E47/RR transfectant (10/11)# | c att gtg | cac agt g | |
| E47/RR transfectant (1/11)# | c att gtg CCT TCC TAC | CT GGG GGA TAC G cac agt g | |
| HEB/RR transfectant (7/7)# | c att gtg | cac agt g | |
| Thymus | c att gtg CCT TCC TAC | N region | T GGG GGA TAC G cac agt g |
| c att gtg CCT TCC TA | ACT GGG GGA TAC G cac agt g | ||
| c att gtg | cac agt g | ||
| Dδ2-Dδ3 excision circles‡ | |||
| Germline2-154 | c act gtg | cac aca g | |
| E12/RR transfectant | c act gtg ACT GGG GGA | T TCC TAC cac aca g | |
| E47/RR transfectant | c act gtg ACT GGG GGA TAC G | C TAC cac aca g | |
| HEB/RR transfectant | c act gtg ACT GGG GGA | TAC cac aca g | |
| c act gtg ACT GGG GGA TAC | cac aca g | ||
| Thymus | c act gtg | cac aca g | |
| c act gtg | N region | cac aca g | |
| c act gtg | N region | AC cac aca g | |
| c act | N region | cac aca g |
| Sample . | 5′ gene segment* . | P nucleotides . | 3′ gene segment* . |
|---|---|---|---|
| Vδ2-Dδ3 rearrangements | |||
| Germline† | TGT GCC TGT GAC ACC | ACT GGG GGA TAC G cac agt g | |
| E47/RR transfectant | 0 | −3 | |
| 0 | −4 | ||
| HEB/RR transfectant | −2 | −3 | |
| −1 | −2 | ||
| −1 | −3 | ||
| −2 | T | 0 | |
| 0 | −3 | ||
| Dδ2-Dδ3 rearrangements‡ | |||
| Germline2-153 | c att gtg CCT TCC TAC | ACT GGG GGA TAC G cac agt g | |
| E12/RR transfectant (4/4)# | c att gtg | cac agt g | |
| E47/RR transfectant (10/11)# | c att gtg | cac agt g | |
| E47/RR transfectant (1/11)# | c att gtg CCT TCC TAC | CT GGG GGA TAC G cac agt g | |
| HEB/RR transfectant (7/7)# | c att gtg | cac agt g | |
| Thymus | c att gtg CCT TCC TAC | N region | T GGG GGA TAC G cac agt g |
| c att gtg CCT TCC TA | ACT GGG GGA TAC G cac agt g | ||
| c att gtg | cac agt g | ||
| Dδ2-Dδ3 excision circles‡ | |||
| Germline2-154 | c act gtg | cac aca g | |
| E12/RR transfectant | c act gtg ACT GGG GGA | T TCC TAC cac aca g | |
| E47/RR transfectant | c act gtg ACT GGG GGA TAC G | C TAC cac aca g | |
| HEB/RR transfectant | c act gtg ACT GGG GGA | TAC cac aca g | |
| c act gtg ACT GGG GGA TAC | cac aca g | ||
| Thymus | c act gtg | cac aca g | |
| c act gtg | N region | cac aca g | |
| c act gtg | N region | AC cac aca g | |
| c act | N region | cac aca g |
RSS indicates recombination signal sequence; RR, RAG1 plus RAG2.
Indicated are the number of nucleotides deleted at the 5′ and 3′ gene segments. Lower case represents RSS nucleotides; upper case, nucleotides of gene segments.
For 5′ gene segment, this is Vδ2 (coding); for 3′ gene segment, this is Dδ3 (coding + 3′ RSS).
See Figure 2 for the Dδ2-Dδ3 rearrangement and excision circle composition, such as they occur in the classical and alternative recombination mechanisms.
For 5′ gene segment, this is Dδ2 (5′ RSS + coding); for 3′ gene segment, this is Dδ3 (coding + 3′ RSS).
For 5′ gene segment, this is Dδ3 (5′ RSS); for 3′ gene segment, this is Dδ2 (3′ RSS).
#Indicated are the number of PCR products of the total number of sequenced PCR products.