Surface phenotypes of fetal liver cell lines under different culture conditions
| Cell types and conditions . | Percentage of cells positive . | |||
|---|---|---|---|---|
| B220 . | Gr-1 . | CD11b . | F4/80 . | |
| FL-1(+/−) SCF, IL-3 | 95 ± 2 | 30 ± 10 | 5 ± 1 | 2 ± 1 |
| FL-1(+/−) GM-CSF | 63 ± 3 | 67 ± 7 | 51 ± 5 | 3 ± 1 |
| FL-1(+/−) SCF, IL-3 + ATRA | 94 ± 2 | 34 ± 6 | 4 ± 2 | ND |
| FL-8(−/−) SCF, IL-3 | 37 ± 2 | 35 ± 3 | 45 ± 3 | 7 ± 2 |
| FL-8(−/−) GM-CSF | 11 ± 3 | 92 ± 2 | 91 ± 3 | 2 ± 1 |
| FL-8(−/−) SCF, IL-3 + ATRA | 35 ± 1 | 47 ± 5 | 65 ± 3 | ND |
| Cell types and conditions . | Percentage of cells positive . | |||
|---|---|---|---|---|
| B220 . | Gr-1 . | CD11b . | F4/80 . | |
| FL-1(+/−) SCF, IL-3 | 95 ± 2 | 30 ± 10 | 5 ± 1 | 2 ± 1 |
| FL-1(+/−) GM-CSF | 63 ± 3 | 67 ± 7 | 51 ± 5 | 3 ± 1 |
| FL-1(+/−) SCF, IL-3 + ATRA | 94 ± 2 | 34 ± 6 | 4 ± 2 | ND |
| FL-8(−/−) SCF, IL-3 | 37 ± 2 | 35 ± 3 | 45 ± 3 | 7 ± 2 |
| FL-8(−/−) GM-CSF | 11 ± 3 | 92 ± 2 | 91 ± 3 | 2 ± 1 |
| FL-8(−/−) SCF, IL-3 + ATRA | 35 ± 1 | 47 ± 5 | 65 ± 3 | ND |
Fetal liver cell lines cultured under the indicated conditions were harvested, incubated with phycoerythrin or fluorescein isothiocyanate–conjugated antibodies to the indicated antigens, and subjected to FACS analysis. For GM-CSF induction, cells continuously growing in SCF and IL-3 were washed, resuspended in GM-CSF alone, and cultured for an additional 4 days. ATRA induction (10 μM) was for 5 days.
ND indicates not determined.