Table 3.

Phenotypic markers of the 2 independent transformed rabbit T-cell lines

Antibody3-150Cell line (6 mo) C2180LNCell line (6 mo) C2174PBMCCell line (20 mo) C2174PBMCNormal rabbit PBMCs
CD3 2.7 22.12 84 39.4 
CD4 42.2 18.1 79 57.4 
CD8 2.2 16.58 75 42 
CD25 47 95.4 94 ND 
CD11a 34 19.5 90 ND 
CD11b 0.17 7.8 0.1 ND 
CD5 23 5.75 0.3 ND  
IgM 0.81 2.1 0 ND 
CD4/CD8 ND ND 37.153-151 ND 
CD3/CD4 ND ND 52.33 ND 
CD8/CD3 ND ND 17.213-151 ND  
LMP 1 ND ND 50 ND  
LMP 1/CD3 ND ND 54.74 ND 
LMP 1/CD4 ND ND 62.72 ND  
LMP 1/CD8 ND ND 50.33 ND 
Antibody3-150Cell line (6 mo) C2180LNCell line (6 mo) C2174PBMCCell line (20 mo) C2174PBMCNormal rabbit PBMCs
CD3 2.7 22.12 84 39.4 
CD4 42.2 18.1 79 57.4 
CD8 2.2 16.58 75 42 
CD25 47 95.4 94 ND 
CD11a 34 19.5 90 ND 
CD11b 0.17 7.8 0.1 ND 
CD5 23 5.75 0.3 ND  
IgM 0.81 2.1 0 ND 
CD4/CD8 ND ND 37.153-151 ND 
CD3/CD4 ND ND 52.33 ND 
CD8/CD3 ND ND 17.213-151 ND  
LMP 1 ND ND 50 ND  
LMP 1/CD3 ND ND 54.74 ND 
LMP 1/CD4 ND ND 62.72 ND  
LMP 1/CD8 ND ND 50.33 ND 
F3-150

These antibodies recognize specifically only rabbit markers.

F3-151

CD8 down-regulation occurred by double staining with permeabilized cells with αCD3 and αCD4 antibodies but not with LMP1 antibodies.

ND indicates not done.

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