Table 1. The effects of hematopoietic... | American Society of Hematology

Table 1.

The effects of hematopoietic growth factors on hypodiploid DNA content and survivin protein expression in umbilical cord blood CD34⁺ cells

CD34⁺cells	Tpo + FL + SCF
CD34⁺cells	−	+
% cells with hypodiploid DNA^*	38.5	16.8
Survivin^†	115.5	507.4

CD34⁺cells	Tpo + FL + SCF
% cells with hypodiploid DNA^*	38.5	16.8
Survivin^†	115.5	507.4

Umbilical cord blood CD34⁺ cells were cultured in the absence or presence of 100 ng/mL each of Tpo, FL, and SCF for 48 hours. Data are from 1 of 2 identical experiments.

Tpo indicates thrombopoietin; FL, Flt3 ligand; SCF, stem cell factor.

Hypodiploid DNA content was determined by flow cytometry on cells stained with 0.5 μg/mL propidium iodide at 48 hours.

†

Survivin expression was determined by intracellular staining with 5 μg/mL polyclonal antisurvivin and flow cytometry at 48 hours. Data are presented as mean channel fluorescence.

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