Hematopoietic reconstitution by Ly-5.1+ cells separated from the muscle cells of chimeric mice
| Experiment no.5-150 . | Cells transplanted5-151 . | Radioprotective cells5-152 . | Secondary recipients (no. recipients) . | % Chimerism in secondary recipients' PB . | Multilineage reconstitution by designated muscle cells5-153 . | ||
|---|---|---|---|---|---|---|---|
| G/M . | T . | B . | |||||
| 7 | Ly-5.1+ 1.0 × 105 | Ly-5.2 BM cells 2 × 105 | Ly-5.2 (2) | 1 | 24 | 89 | 8 |
| 1 | 21 | 64 | 14 | ||||
| Ly-5.1−5.0 × 105 | Ly-5.1 BM cells 2 × 105 | Ly-5.1 (2) | 0 | 0 | 0 | 0 | |
| 0 | ND | ND | ND | ||||
| 8 | Ly-5.1+ 8.0 × 104 | Ly-5.2 BM cells 2 × 105 | Ly-5.2 (2) | 9 | 18 | 86 | 3 |
| 5 | 18 | 87 | 5 | ||||
| Ly-5.1− 3.4 × 105 | Ly-5.1 BM cells 2 × 105 | Ly-5.1 (2) | 0 | 0 | 0 | 0 | |
| 0 | ND | ND | ND | ||||
| Experiment no.5-150 . | Cells transplanted5-151 . | Radioprotective cells5-152 . | Secondary recipients (no. recipients) . | % Chimerism in secondary recipients' PB . | Multilineage reconstitution by designated muscle cells5-153 . | ||
|---|---|---|---|---|---|---|---|
| G/M . | T . | B . | |||||
| 7 | Ly-5.1+ 1.0 × 105 | Ly-5.2 BM cells 2 × 105 | Ly-5.2 (2) | 1 | 24 | 89 | 8 |
| 1 | 21 | 64 | 14 | ||||
| Ly-5.1−5.0 × 105 | Ly-5.1 BM cells 2 × 105 | Ly-5.1 (2) | 0 | 0 | 0 | 0 | |
| 0 | ND | ND | ND | ||||
| 8 | Ly-5.1+ 8.0 × 104 | Ly-5.2 BM cells 2 × 105 | Ly-5.2 (2) | 9 | 18 | 86 | 3 |
| 5 | 18 | 87 | 5 | ||||
| Ly-5.1− 3.4 × 105 | Ly-5.1 BM cells 2 × 105 | Ly-5.1 (2) | 0 | 0 | 0 | 0 | |
| 0 | ND | ND | ND | ||||
Ly-5.1+ cells or Ly-5.1− cells separated from the muscle of chimeric mice were transplanted into lethally irradiated secondary recipient mice along with radioprotective cells. At 2 months later, PB was collected from the secondary recipients, and the levels of engraftment were analyzed by flow cytometry. Nucleated PB cells were stained with anti-Ly-5.1 or anti-Ly-5.2 and either anti-Gr-1 and anti-Mac-1, anti-Thy-1.2, or anti-B220.
PB indicates peripheral blood; G/M, granulocytes and/or monocytes; T, T lymphocytes; B, B lymphocytes; ND, not determined.
The experimental mice correspond to experiments 7 and 8 in Table 3.
The numbers of cells transplanted per mouse are in proportion to the numbers of Ly-5.1+ cells and Ly-5.1− cells in the mononuclear cells harvested from the muscle of chimeric mice.
Unfractionated BM cells were used as radioprotective cells.
Numbers indicate the percentages of cells within each lineage that expressed Ly-5.1 (for Ly-5.2 recipients) or Ly-5.2 (for Ly-5.1 recipients).