The NFAT inhibitor GFP-VIVIT suppresses IFN-γ production by T cells
. | GFP . | GFP-VIVIT . | % inhibition by VIVIT . | ||||
---|---|---|---|---|---|---|---|
% IFN-γ–pos . | MFI . | % pos × MFI . | % IFN-γ–pos . | MFI . | % pos × MFI . | % pos × MFI . | |
Experiment 1 | 61.1 | 243.9 | 14 893 | 23.7 | 108.6 | 2 573 | 82.7 |
Experiment 2 | 58.9 | 251.4 | 14 797 | 14.9 | 77.3 | 1 155 | 92.2 |
Experiment 3 | 33.2 | 86.6 | 2 871 | 8.3 | 63.0 | 520 | 81.9 |
. | GFP . | GFP-VIVIT . | % inhibition by VIVIT . | ||||
---|---|---|---|---|---|---|---|
% IFN-γ–pos . | MFI . | % pos × MFI . | % IFN-γ–pos . | MFI . | % pos × MFI . | % pos × MFI . | |
Experiment 1 | 61.1 | 243.9 | 14 893 | 23.7 | 108.6 | 2 573 | 82.7 |
Experiment 2 | 58.9 | 251.4 | 14 797 | 14.9 | 77.3 | 1 155 | 92.2 |
Experiment 3 | 33.2 | 86.6 | 2 871 | 8.3 | 63.0 | 520 | 81.9 |
Table summarizing the information of 3 independent experiments as shown in Figure 7B. For each experiment, the first column shows the percentage of IFN-γ–producing cells in the cell populations expressing high levels of GFP and GFP-VIVIT (number of cells in upper right quadrant divided by number of cells in upper and lower right quadrants), and the second column shows the mean fluorescence intensity (MFI) of IFN-γ staining in the cells in the upper right quadrant. Note that only cells expressing high levels of GFP and GFP-VIVIT (fluorescence cutoff 100) are considered for reasons described in “Results.” The product of these 2 numbers is shown in the last column and is a measure of total IFN-γ production by the GFP- or GFP-VIVIT–expressing cells. Because the transfection efficiencies for GFP and GFP-VIVIT were comparable, the ratio of the numbers in the last column is a measure of the ability of VIVIT to inhibit IFN-γ production. The same calculations performed on the untransfected (GFP-negative) cells showed no inhibition by VIVIT.