Effect of bcl-2 antisense oligonucleotides on Bcl-2 protein expression and IL-7–mediated promotion of viability in T-ALL cells
| . | Percentage of live cells . | Bcl-2 protein expression (mean fluorescence intensity) . |
|---|---|---|
| Media | 34.21 | 6.96 |
| IL-7 | 48.64 | 12.25 |
| IL-7 and control oligonucleotide | 51.83 | 14.26 |
| IL-7 and bcl-2 antisense | 34.08 | 5.01 |
| . | Percentage of live cells . | Bcl-2 protein expression (mean fluorescence intensity) . |
|---|---|---|
| Media | 34.21 | 6.96 |
| IL-7 | 48.64 | 12.25 |
| IL-7 and control oligonucleotide | 51.83 | 14.26 |
| IL-7 and bcl-2 antisense | 34.08 | 5.01 |
T-cell acute lymphoblastic leukemia (T-ALL) cells were cultured with media, IL-7, or IL-7 and Bcl-2 antisense or control oligonucleotides. Bcl-2 protein expression and percentage of viable cells were examined in 6 patients, and results of one representative experiment are shown. The effect of bcl-2 antisense oligonucleotide was examined in 2 patients, and results of one experiment are shown.