Ability to induce in vitro anti-β2GPI antibody production from autologous B cells and cytokine profiles in 12 β2GPI-specific CD4+ T-cell clones
| T-cell clones . | Anti-β2GPI antibody levels in supernatants (OD450)3-150 . | Cytokine levels (pg/mL)3-151 . | |||
|---|---|---|---|---|---|
| IFN-γ . | IL-4 . | IL-6 . | IL-10 . | ||
| Patient O.M. | |||||
| OM2 | 0.590 ± 0.022 | 880 ± 18 | > 500 | 487 ± 27 | 525 ± 30 |
| OM3 | 0.297 ± 0.014 | 1248 ± 22 | 191 ± 10 | 132 ± 7 | < 1 |
| OM7 | 0.450 ± 0.034 | 280 ± 11 | 305 ± 9 | 138 ± 5 | 720 ± 18 |
| OM83-152 | 0.016 ± 0.002 | 560 ± 15 | < 1 | < 1 | < 1 |
| Patient E.Y. | |||||
| EY3 | 0.422 ± 0.034 | 380 ± 10 | < 1 | 126 ± 16 | 316 ± 24 |
| EY8 | 0.412 ± 0.024 | 468 ± 9 | < 1 | 326 ± 25 | 461 ± 13 |
| EY93-152 | 0.023 ± 0.003 | 746 ± 18 | 7 ± 1 | < 1 | 262 ± 11 |
| EY12 | 0.396 ± 0.031 | 1632 ± 39 | > 500 | 175 ± 10 | 41 ± 5 |
| Patient K.S. | |||||
| KS3 | 0.400 ± 0.028 | 490 ± 7 | > 500 | 343 ± 25 | 371 ± 17 |
| KS4 | 0.418 ± 0.041 | 1214 ± 32 | 13 ± 7 | 546 ± 5 | 74 ± 11 |
| KS5 | 0.486 ± 0.020 | 824 ± 21 | 286 ± 16 | 366 ± 13 | 626 ± 22 |
| KS7 | 0.209 ± 0.012 | 653 ± 29 | < 1 | 97 ± 4 | 759 ± 30 |
| T-cell clones . | Anti-β2GPI antibody levels in supernatants (OD450)3-150 . | Cytokine levels (pg/mL)3-151 . | |||
|---|---|---|---|---|---|
| IFN-γ . | IL-4 . | IL-6 . | IL-10 . | ||
| Patient O.M. | |||||
| OM2 | 0.590 ± 0.022 | 880 ± 18 | > 500 | 487 ± 27 | 525 ± 30 |
| OM3 | 0.297 ± 0.014 | 1248 ± 22 | 191 ± 10 | 132 ± 7 | < 1 |
| OM7 | 0.450 ± 0.034 | 280 ± 11 | 305 ± 9 | 138 ± 5 | 720 ± 18 |
| OM83-152 | 0.016 ± 0.002 | 560 ± 15 | < 1 | < 1 | < 1 |
| Patient E.Y. | |||||
| EY3 | 0.422 ± 0.034 | 380 ± 10 | < 1 | 126 ± 16 | 316 ± 24 |
| EY8 | 0.412 ± 0.024 | 468 ± 9 | < 1 | 326 ± 25 | 461 ± 13 |
| EY93-152 | 0.023 ± 0.003 | 746 ± 18 | 7 ± 1 | < 1 | 262 ± 11 |
| EY12 | 0.396 ± 0.031 | 1632 ± 39 | > 500 | 175 ± 10 | 41 ± 5 |
| Patient K.S. | |||||
| KS3 | 0.400 ± 0.028 | 490 ± 7 | > 500 | 343 ± 25 | 371 ± 17 |
| KS4 | 0.418 ± 0.041 | 1214 ± 32 | 13 ± 7 | 546 ± 5 | 74 ± 11 |
| KS5 | 0.486 ± 0.020 | 824 ± 21 | 286 ± 16 | 366 ± 13 | 626 ± 22 |
| KS7 | 0.209 ± 0.012 | 653 ± 29 | < 1 | 97 ± 4 | 759 ± 30 |
IgG anti-β2GPI antibody levels were measured in supernatants of β2GPI-specific T-cell clones cultured with autologous B cells in the presence of GP-F. Results are shown in mean ± SD. IgG anti-β2GPI antibody levels in cultures of B cells alone were 0.011 ± 0.001 (O.M.), 0.012 ± 0.002 (E.Y.), and 0.001 ± 0.001 (K.S.).
Individual cytokine levels were measured in culture supernatants of β2GPI-specific T-cell clones stimulated with anti-CD3 mAb and PHA. Results are shown in mean ± SD.
T-cell clones lacking helper activity.