Table 1. Cytogenetic analysis of FA-C... | American Society of Hematology

Table 1.

Cytogenetic analysis of FA-C lymphoblast cell lines

Cell line	No. of metaphases	No. of cells with radials^*	Radials (%)^†
JY	50	1	2.0
HSC536N	53	12	22.6
HSC536N/neo	50	17	34.0
HSC536N/FANCC	50	0	0
HSC536N/F64A	50	1	2.0
HSC536N/T66A	52	2	3.8
HSC536N/S249A	50	2	4.0
HSC536N/E251A	50	1	2.0
HSC536N/F525A	50	0	0
HSC536N/Y531A	50	1	2.0

Cell line	No. of metaphases	No. of cells with radials^*	Radials (%)^†
JY	50	1	2.0
HSC536N	53	12	22.6
HSC536N/neo	50	17	34.0
HSC536N/FANCC	50	0	0
HSC536N/F64A	50	1	2.0
HSC536N/T66A	52	2	3.8
HSC536N/S249A	50	2	4.0
HSC536N/E251A	50	1	2.0
HSC536N/F525A	50	0	0
HSC536N/Y531A	50	1	2.0

Cells were treated with 40 ng/mL MMC for 48 hours and scored for radial formation.

A radial was defined as a chromatid interchange in which chromatids remained paired in mitosis giving rise to a structure with multiple arms.

Greater than 20% radial formation is diagnostic for FA.