American Society of Hematology

Table 1.

Primers and restriction enzyme sites for detection of hot-spot mutations in N- and K-ras genes

Nucleotide positions in codon 12, 13, or 61 targeted for screening are depicted in boxes.

Arrows show the nucleotide targeted for design of the mismatch primer. Uppercase nucleotide in the primer represents the mismatch.

Antisense primer.

Sense primer.