Table 1. CD35, CD11b, and CD18... | American Society of Hematology

Table 1.

CD35, CD11b, and CD18 expression at the surface of PMNs from the 3 patients with GPS

	Mean fluorescence intensity
	PBS 4°C		PBS 37°C		fMLP 37°C
	First	Second	First	Second	First	Second
CD35
Control	15	27	17	41	106	141
Ju	111	123	128	137	104	130
Ma	89	97	97	99	93	117
Me	82	90	94	105	80	104
CD11b
Controls	145 ± 2		183 ± 12		2237 ± 198
Ju	563	638	687	797	812	875
Ma	607	705	806	824	732	862
Me	606	616	804	821	816	864
CD18
Controls	40	20	50	10	400	700
Ju	116	144	131	129	150	181
Ma	138	160	156	186	133	176
Me	125	143	161	180	174	201

	Mean fluorescence intensity
	PBS 4°C		PBS 37°C		fMLP 37°C
	First	Second	First	Second	First	Second
CD35
Control	15	27	17	41	106	141
Ju	111	123	128	137	104	130
Ma	89	97	97	99	93	117
Me	82	90	94	105	80	104
CD11b
Controls	145 ± 2		183 ± 12		2237 ± 198
Ju	563	638	687	797	812	875
Ma	607	705	806	824	732	862
Me	606	616	804	821	816	864
CD18
Controls	40	20	50	10	400	700
Ju	116	144	131	129	150	181
Ma	138	160	156	186	133	176
Me	125	143	161	180	174	201

Whole blood was incubated with FITC-CD35, PE-CD11b, or FITC-CD18 antibodies at 4°C for 30 minutes either after maintaining the samples at 4°C or after incubation with PBS, or fMLP (10⁻⁶mol/L) at 37°C for 5 minutes. Mean fluorescence intensity was recorded as described in “Patients, materials, and methods.” Values obtained with an irrelevant antibody of the same isotype were subtracted. The CD35 and CD18 values of the day controls are in the range of other 6 healthy controls. For CD11b, the values are means ± SEM of 15 healthy controls.

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