Oligonucleotide primer pairs used for amplification and direct sequencing of ABO gene fragments
| Primer designation . | Primer pair . | Primer sequence (5′ → 3′) . | Primer location . | ABO region amplified . | Fragment size* (bp) . |
|---|---|---|---|---|---|
| Pro-F | 137 | ggaaacaaatcctacccctac | 5′-UTR | Enhancer (CBF-NFY) | 215/344 |
| Pro-R | gtgctgcctgtgcctgttac | 5′-UTR | |||
| mo-12F | 2 | ggcgccgtcccttcctag | 5′-UTR | Promoter, exon 1 | 267 |
| mo-12R | cctgcggtagcggctcct | Intron 1 | |||
| mo-21F | 3 | ggtgagagaaggagggtgag | Intron 1 | Exon 2-3 | 935 |
| mo-31R | ccagcaccccggccagca | Intron 3 | |||
| mo-41F | 431 | taaatcctgctcctagactaaac | Intron 3 | Exon 4 | 148 |
| mo-42R | ggacaattctgtgacatgggag | Intron 4 | |||
| mo-51F | 5 | tgcatcccacgctttccatgc | Intron 4 | Exon 5-6 | 823 |
| mo-46R19 | actcgccactgcctgggtctc | Intron 6 | |||
| mo-57a | 6 | gggtttgttcctatctctttgc | Intron 5 | Exon 6-7 | 2273 |
| mo-71R19 | gggcctaggcttcagttactc | 3′-UTR | |||
| ABO-261F-C | 7a | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (non-O1/O1v) | 2002 |
| mo-71R19 | gggcctaggcttcagttactc | 3′-UTR | |||
| ABO-261F-C | 7b | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (A2-specific) | 1888 |
| ABO-1060R-A2 | CCTGGCAGCCGCTCACGGT | Exon 7 | |||
| ABO-261F-C | 7c | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (B-specific) | 1923 |
| ABO-1096R-B | GAGGGGGACGGGGCTGCT | 3′-UTR | |||
| ABO-297F-B† | — | CCATTGTCTGGGAGGGCACG | Exon 6 | — | — |
| ABO-467F-A2† | — | CTATGTCTTCACCGACCAGCT | Exon 7 | — | — |
| ABO-467R-A2‡ | CGGGGCACCGCGGCCAG | Exon 7 | |||
| ABO-526R-B‡ | GCCAGCGCTTGTAGGCGCC | Exon 7 | |||
| ABO-796R-B‡ | CCCCGAAGAACGCCCCCAT | Exon 7 | |||
| ABO-802R-O2‡ | CCGACCCCCCGAAGAACCT | Exon 7 | |||
| ABO-803R-B‡ | ACCGACCCCCCGAAGAACG | Exon 7 |
| Primer designation . | Primer pair . | Primer sequence (5′ → 3′) . | Primer location . | ABO region amplified . | Fragment size* (bp) . |
|---|---|---|---|---|---|
| Pro-F | 137 | ggaaacaaatcctacccctac | 5′-UTR | Enhancer (CBF-NFY) | 215/344 |
| Pro-R | gtgctgcctgtgcctgttac | 5′-UTR | |||
| mo-12F | 2 | ggcgccgtcccttcctag | 5′-UTR | Promoter, exon 1 | 267 |
| mo-12R | cctgcggtagcggctcct | Intron 1 | |||
| mo-21F | 3 | ggtgagagaaggagggtgag | Intron 1 | Exon 2-3 | 935 |
| mo-31R | ccagcaccccggccagca | Intron 3 | |||
| mo-41F | 431 | taaatcctgctcctagactaaac | Intron 3 | Exon 4 | 148 |
| mo-42R | ggacaattctgtgacatgggag | Intron 4 | |||
| mo-51F | 5 | tgcatcccacgctttccatgc | Intron 4 | Exon 5-6 | 823 |
| mo-46R19 | actcgccactgcctgggtctc | Intron 6 | |||
| mo-57a | 6 | gggtttgttcctatctctttgc | Intron 5 | Exon 6-7 | 2273 |
| mo-71R19 | gggcctaggcttcagttactc | 3′-UTR | |||
| ABO-261F-C | 7a | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (non-O1/O1v) | 2002 |
| mo-71R19 | gggcctaggcttcagttactc | 3′-UTR | |||
| ABO-261F-C | 7b | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (A2-specific) | 1888 |
| ABO-1060R-A2 | CCTGGCAGCCGCTCACGGT | Exon 7 | |||
| ABO-261F-C | 7c | AGGAAGGATGTCCTCGTGGTG | Exon 6 | Exon 7 (B-specific) | 1923 |
| ABO-1096R-B | GAGGGGGACGGGGCTGCT | 3′-UTR | |||
| ABO-297F-B† | — | CCATTGTCTGGGAGGGCACG | Exon 6 | — | — |
| ABO-467F-A2† | — | CTATGTCTTCACCGACCAGCT | Exon 7 | — | — |
| ABO-467R-A2‡ | CGGGGCACCGCGGCCAG | Exon 7 | |||
| ABO-526R-B‡ | GCCAGCGCTTGTAGGCGCC | Exon 7 | |||
| ABO-796R-B‡ | CCCCGAAGAACGCCCCCAT | Exon 7 | |||
| ABO-802R-O2‡ | CCGACCCCCCGAAGAACCT | Exon 7 | |||
| ABO-803R-B‡ | ACCGACCCCCCGAAGAACG | Exon 7 |
Primer sequences corresponding to untranslated sequences are shown in lower case.
The exact number of nucleotides may vary slightly between different alleles.
Used as sequencing primers only.
Each primer was used with ABO-261F-C for coupling of mutations in exons 6 and 7.36 The nucleotide- and allele-specificity is indicated by the name of the primers.