NE and CG cleave VCAM-1 but neither VLA-4, VLA-5, nor PECAM-1
| Antigens . | No treatment . | Neutrophil-CM . | NE . | CG . | NE + CG . |
|---|---|---|---|---|---|
| VCAM-1 | 39.6 | 6.4* | 6.2* | 12.7* | 5.6* |
| VLA-4 | 186.8 | 190.6 | 183.3 | 173.3 | 184.4 |
| VLA-5 | 111.6 | 115.8 | 99.8 | 101.2 | 98.1 |
| PECAM-1 | 153.9 | 158.1 | 134.5 | 149.0 | 135.9 |
| Antigens . | No treatment . | Neutrophil-CM . | NE . | CG . | NE + CG . |
|---|---|---|---|---|---|
| VCAM-1 | 39.6 | 6.4* | 6.2* | 12.7* | 5.6* |
| VLA-4 | 186.8 | 190.6 | 183.3 | 173.3 | 184.4 |
| VLA-5 | 111.6 | 115.8 | 99.8 | 101.2 | 98.1 |
| PECAM-1 | 153.9 | 158.1 | 134.5 | 149.0 | 135.9 |
FDCP1-huVCAM-1 (VCAM-1 staining) and MO7e cells (VLA-4, VLA-5, and PECAM-1 staining) were incubated for 1 hour at 37°C in the presence of neutrophil-conditioned medium, NE, or CG and were labeled with specific mAbs before analysis by flow cytometry. Settings were optimized to obtain MFI values between 1.4 and 1.6 with nonbinding isotype-matched control antibodies. Values are expressed as MFI and significant differences with untreated cells (P < .01, n = 3) are shown with an asterisk. CM indicates conditioned medium.