IFN-γ production by bcr-abl-specific CD4+T-lymphocyte clones in response to stimulation with synthetic peptides
| Clone . | Peptide stimulation . | mAb added . | IFN-γ production, pg/mL* . |
|---|---|---|---|
| MY-1 | None | None | < 10 |
| b3b4 | None | < 10 | |
| 1A-a2 | None | < 10 | |
| b2a2 | None | < 10 | |
| b3a2 | None | 1789 ± 158 | |
| Anti-HLA class I | 1808 ± 132 | ||
| Anti-HLA-DR | 298 ± 28 | ||
| TO-1 | None | None | < 10 |
| b3b4 | None | < 10 | |
| 1A-a2 | None | < 10 | |
| b2a2 | None | < 10 | |
| b3a2 | None | 1815 ± 170 | |
| Anti-HLA class I | 1636 ± 139 | ||
| Anti-HLA-DR | 193 ± 38 |
| Clone . | Peptide stimulation . | mAb added . | IFN-γ production, pg/mL* . |
|---|---|---|---|
| MY-1 | None | None | < 10 |
| b3b4 | None | < 10 | |
| 1A-a2 | None | < 10 | |
| b2a2 | None | < 10 | |
| b3a2 | None | 1789 ± 158 | |
| Anti-HLA class I | 1808 ± 132 | ||
| Anti-HLA-DR | 298 ± 28 | ||
| TO-1 | None | None | < 10 |
| b3b4 | None | < 10 | |
| 1A-a2 | None | < 10 | |
| b2a2 | None | < 10 | |
| b3a2 | None | 1815 ± 170 | |
| Anti-HLA class I | 1636 ± 139 | ||
| Anti-HLA-DR | 193 ± 38 |
The results shown represent the means ± SDs of triplicate experiments.
IFN-γ production by MY-1 and TO-1 in the presence of autologous APCs with and without various synthetic peptides and with and without anti-HLA mAb was determined by ELISA.