Table 1. Reverse... | American Society of Hematology

Table 1.

Reverse transcriptase–polymerase chain reaction primers and probes

Qualitative RT-PCR

First-round RT-PCR

CMLNA

5′TGGAGCTGCAGATGCTGACCAACTCG3′

CMLNB

5′ATCTCCACTGGCCACAAAATCATACA3′

Second-round nested PCR

CMLNH

5′GATCTCCTCTGACTATGAGCGTCCA3′

CMLNI

5′-TTCACCTTTAGTTATGCTTAGAGTG3′

Quantitative RT-PCR (Q-PCR)

bcr-abl b2a2 primers

BCRTM2

5′CAT TCC GCT GAC CAT CAA TAA3′

ABLTM

5′AAC GAG CGG CTT CAC TCA GA3′

bcr-abl b3a2 primers

BCRTM3

5′CCA CTG GAT TTA AGC AGA GTT CA3′

ABLTM

5′AAC GAG CGG CTT CAC TCA GA3′

bcr-ablprobe

ABLTMPROBE

5′AGCGGCCAGTAGCATCTGACTTTGAGC3′

β2-microglobulin primers

β2TMF

5′CAT TCG GGC CGA GAT GTC3′

β2TMR

5′CTC CAG GCC AGA AAG AGA GAG TAG3′

β2-microglobulin probe

β2TMPROBE

5′CCG TGG CCT TAG CTG TGC TCG C3′