Table 1.

Cell surface levels of integrins, tTG, and FXIIIA on untreated and TPA-treated THP-1 cells

Treatmentβ1-integrin*β2-integrinβ3-integrintTG1-153FXIIIA1-155
−TPA 178.5 126.0 31.8 18.3 113.6 
+TPA 228.1 165.3 118.2 166.6 26.8 
Treatmentβ1-integrin*β2-integrinβ3-integrintTG1-153FXIIIA1-155
−TPA 178.5 126.0 31.8 18.3 113.6 
+TPA 228.1 165.3 118.2 166.6 26.8 

Live nonpermeabilized untreated (−TPA) and TPA-treated (+TPA) THP-1 cells plated on Fn for 72 hours were analyzed. Values are mean fluorescence intensities from 3 separate experiments.

*

Fluorescence intensity measured with mAb JB1A.

Fluorescence intensity measured with mAb YFC118.3.

Fluorescence intensity measured with mAb B3A.

F1-153

Fluorescence intensity measured with polyclonal anti-tTG antibody.

F1-155

Fluorescence intensity measured with polyclonal antibody to FXIIIA.

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