Cell surface levels of integrins, tTG, and FXIIIA on untreated and TPA-treated THP-1 cells
Treatment . | β1-integrin* . | β2-integrin† . | β3-integrin‡ . | tTG1-153 . | FXIIIA1-155 . |
---|---|---|---|---|---|
−TPA | 178.5 | 126.0 | 31.8 | 18.3 | 113.6 |
+TPA | 228.1 | 165.3 | 118.2 | 166.6 | 26.8 |
Treatment . | β1-integrin* . | β2-integrin† . | β3-integrin‡ . | tTG1-153 . | FXIIIA1-155 . |
---|---|---|---|---|---|
−TPA | 178.5 | 126.0 | 31.8 | 18.3 | 113.6 |
+TPA | 228.1 | 165.3 | 118.2 | 166.6 | 26.8 |
Live nonpermeabilized untreated (−TPA) and TPA-treated (+TPA) THP-1 cells plated on Fn for 72 hours were analyzed. Values are mean fluorescence intensities from 3 separate experiments.
Fluorescence intensity measured with mAb JB1A.
Fluorescence intensity measured with mAb YFC118.3.
Fluorescence intensity measured with mAb B3A.
Fluorescence intensity measured with polyclonal anti-tTG antibody.
Fluorescence intensity measured with polyclonal antibody to FXIIIA.