PI/Annexin FACS Analysis from total bone marrow samples
| Genotype . | n . | % Staining . | |
|---|---|---|---|
| Annexin+ PI− . | Annexin+PI+ . | ||
| Fancc+/−Sod1+/− | 5 | 25.49 ± 4.59 | 15.52 ± 3.08 |
| Fancc−/−Sod1−/− | 5 | 28.38 ± 3.83 | 16.44 ± 6.12 |
| Genotype . | n . | % Staining . | |
|---|---|---|---|
| Annexin+ PI− . | Annexin+PI+ . | ||
| Fancc+/−Sod1+/− | 5 | 25.49 ± 4.59 | 15.52 ± 3.08 |
| Fancc−/−Sod1−/− | 5 | 28.38 ± 3.83 | 16.44 ± 6.12 |
FACS Staining was used to quantitate the number of apoptotic cells from total BM samples of 8-9 week old mice. Annexin+/PI− values represent cells initiating the apoptotic process and Annexin+/PI+ values represent cells that have already undergone apoptosis and death.