Effect of anti-CD80 or -CD86 antibodies on cytokine release by dendritic cell–activated T cells
| . | Unpulsed DCs . | Der p 1– pulsed DCs . | Der p 1– pulsed DCs + anti-CD80 . | Der p 1– pulsed DCs + anti-CD86 . | Der p 1– pulsed DCs + anti-CD80 and anti-CD86 . |
|---|---|---|---|---|---|
| House dust mite– sensitive patients | |||||
| IL-4 | 120 ± 53 | 480 ± 58.1 | 386.1 ± 78.1 | 145.9 ± 36.8* | 162.6 ± 29.4* |
| IFN-γ | 50.3 ± 18.1 | 80 ± 28.2 | 70.1 ± 15.2 | 62 ± 9.4 | 60.3 ± 10.6 |
| Healthy donors | |||||
| IL-4 | 33.4 ± 17.3 | 17.6 ± 5.7 | 41.8 ± 10.1 | 33.9 ± 15.3 | 38.2 ± 10.2 |
| IFN-γ | 352.9 ± 88.2 | 1832.9 ± 117.6 | 142.7 ± 37.6* | 1695.4 ± 207.2 | 92.9 ± 30.1* |
| . | Unpulsed DCs . | Der p 1– pulsed DCs . | Der p 1– pulsed DCs + anti-CD80 . | Der p 1– pulsed DCs + anti-CD86 . | Der p 1– pulsed DCs + anti-CD80 and anti-CD86 . |
|---|---|---|---|---|---|
| House dust mite– sensitive patients | |||||
| IL-4 | 120 ± 53 | 480 ± 58.1 | 386.1 ± 78.1 | 145.9 ± 36.8* | 162.6 ± 29.4* |
| IFN-γ | 50.3 ± 18.1 | 80 ± 28.2 | 70.1 ± 15.2 | 62 ± 9.4 | 60.3 ± 10.6 |
| Healthy donors | |||||
| IL-4 | 33.4 ± 17.3 | 17.6 ± 5.7 | 41.8 ± 10.1 | 33.9 ± 15.3 | 38.2 ± 10.2 |
| IFN-γ | 352.9 ± 88.2 | 1832.9 ± 117.6 | 142.7 ± 37.6* | 1695.4 ± 207.2 | 92.9 ± 30.1* |
Monocyte-derived DCs from house dust mite–sensitive patients or from healthy donors were pulsed or not with 100 ng/mL Der p 1 for 24 hours. Der p 1–pulsed or unpulsed DCs were then incubated for 2 hours with anti-CD80, anti-CD86, or both antibodies before addition of autologous T cells for 24 hours. Supernatants were harvested, and the amounts of IL-4 and IFN-γ were measured by specific ELISA. Results are expressed as the mean concentration (pg/mL) ± SEM.
P < .05 as compared with Der p 1–pulsed DCs. Italicized entries indicate an effect by the antibody.