Analysis of tumor-derived VH genes
| Case no. . | Patient . | Clone . | VH family . | Gem line V gene . | Homology (%) . | R/S CDR2 . | Mutation FW3 . | D segment . | JH segment . | Tumor-derived clones/clones sequenced . | Frame . | CDR2 R:S ratio . | FW3 R:S ratio . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | PE | M4 | VH3 | 3 -30 | 90 | 4/1 | 6/3 | — | JH6 | 10/14 | In | 4 | 2 |
| 2 | GM | M5 | VH2 | 2 -26 | 91 | 4/0 | 5/3 | 3 -10 | — | 11/13 | In | 4 | 1.6 |
| 3 | BA | M7 | VH4 | 4-59/4-61 | 100 | — | — | 3 -3 | JH6c | 12/13 | In | — | — |
| 4 | ML | M14-27 | VH4 | 4 -39 | 94 | 1/1 | 4/3 | (1 -26) | — | 26/28 | In | 1 | 1.3 |
| 5 | FMC | M15 | VH4 | 4 -34 | 83 | 7/1 | 10/7 | (1 -14) | JH4a/b | 11/11 | In | 7 | 1.4 |
| 6 | CG | M19 | VH3 | 3 -23 | 100 | — | — | (6 -25) | JH4d | 7/7 | In | — | — |
| 7 | JS | M20-28 | VH3 | 3 -15 | 93 | 2/3 | 4/2 | 6 -19 | — | 25/26 | In | 0.7 | 2 |
| 8 | ON | M22 | VH3 | 3 -48 | 96 | 4/0 | 2/0 | (1-1/1-20) | — | 15/15 | In | 4 | 2 |
| Case no. . | Patient . | Clone . | VH family . | Gem line V gene . | Homology (%) . | R/S CDR2 . | Mutation FW3 . | D segment . | JH segment . | Tumor-derived clones/clones sequenced . | Frame . | CDR2 R:S ratio . | FW3 R:S ratio . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | PE | M4 | VH3 | 3 -30 | 90 | 4/1 | 6/3 | — | JH6 | 10/14 | In | 4 | 2 |
| 2 | GM | M5 | VH2 | 2 -26 | 91 | 4/0 | 5/3 | 3 -10 | — | 11/13 | In | 4 | 1.6 |
| 3 | BA | M7 | VH4 | 4-59/4-61 | 100 | — | — | 3 -3 | JH6c | 12/13 | In | — | — |
| 4 | ML | M14-27 | VH4 | 4 -39 | 94 | 1/1 | 4/3 | (1 -26) | — | 26/28 | In | 1 | 1.3 |
| 5 | FMC | M15 | VH4 | 4 -34 | 83 | 7/1 | 10/7 | (1 -14) | JH4a/b | 11/11 | In | 7 | 1.4 |
| 6 | CG | M19 | VH3 | 3 -23 | 100 | — | — | (6 -25) | JH4d | 7/7 | In | — | — |
| 7 | JS | M20-28 | VH3 | 3 -15 | 93 | 2/3 | 4/2 | 6 -19 | — | 25/26 | In | 0.7 | 2 |
| 8 | ON | M22 | VH3 | 3 -48 | 96 | 4/0 | 2/0 | (1-1/1-20) | — | 15/15 | In | 4 | 2 |
NA indicates not assessed.