PDs and cloning efficiency of transduced T subclones
| . | Cloning step . | |||||
|---|---|---|---|---|---|---|
| III . | IV . | V . | VI . | VII . | VIII . | |
| Subclones transduced with GFP | ||||||
| G11.8 | ||||||
| PDs | 69 | 89 | 108 | — | ||
| Cloning eff | 65% | 13% | 3% | 0% | ||
| G11.1 | ||||||
| PDs | 68 | 88 | 107 | — | ||
| Cloning eff | 63% | 10% | 13% | 2% | ||
| Subclones transduced with hTERT/GFP | ||||||
| T11.9 | ||||||
| PDs | 69 | 90 | 111 | 133 | 154 | 175 |
| Cloning eff | 30% | 28% | 45% | 16% | 20% | 13% |
| T11.14 | ||||||
| PDs | 68 | 88 | 106 | 125 | — | |
| Cloning eff | 40% | 16% | 10% | 5% | 2% | |
| T11.7 | ||||||
| PDs | 72 | 94 | 115 | 135 | 155 | 175 |
| Cloning eff | 42% | 12% | 19% | 5% | 11% | 20% |
| T11.10 | ||||||
| PDs | 71 | 93 | 114 | 135 | ||
| Cloning eff | 12% | 17% | 8% | 23% | ||
| . | Cloning step . | |||||
|---|---|---|---|---|---|---|
| III . | IV . | V . | VI . | VII . | VIII . | |
| Subclones transduced with GFP | ||||||
| G11.8 | ||||||
| PDs | 69 | 89 | 108 | — | ||
| Cloning eff | 65% | 13% | 3% | 0% | ||
| G11.1 | ||||||
| PDs | 68 | 88 | 107 | — | ||
| Cloning eff | 63% | 10% | 13% | 2% | ||
| Subclones transduced with hTERT/GFP | ||||||
| T11.9 | ||||||
| PDs | 69 | 90 | 111 | 133 | 154 | 175 |
| Cloning eff | 30% | 28% | 45% | 16% | 20% | 13% |
| T11.14 | ||||||
| PDs | 68 | 88 | 106 | 125 | — | |
| Cloning eff | 40% | 16% | 10% | 5% | 2% | |
| T11.7 | ||||||
| PDs | 72 | 94 | 115 | 135 | 155 | 175 |
| Cloning eff | 42% | 12% | 19% | 5% | 11% | 20% |
| T11.10 | ||||||
| PDs | 71 | 93 | 114 | 135 | ||
| Cloning eff | 12% | 17% | 8% | 23% | ||
PDs indicates population doublings; cloning eff, cloning efficiency.