Top 30 increased eosinophil-specific transcripts
| Accession number* . | Name of transcript (protein) . | Eo (%AD†) . | MCs (%AD) . | MNC (%AD) . | Neu (%AD) . | Fold change‡ . |
|---|---|---|---|---|---|---|
| X55988 | Eosinophil-derived neurotoxin | 86.8 | 0.2 | 7.3 | 0.5 | 32.2 |
| L01664 | Charcot-Leyden crystal protein | 78.0 | < 0.1 | 9.2 | 0.2 | 24.5 |
| X81479 | Glycoprotein EMR1 hormone receptor | 59.1 | 1.7 | 5.2 | 2.8 | 18.4 |
| M84526 | Complement factor D | 45.9 | 0.9 | 10.9 | 4.8 | 8.3 |
| J04130 | Macrophage inflammatory protein-1 β | 36.8 | < 0.1 | 6.0 | 1.9 | 14.0 |
| D90144 | Macrophage inflammatory protein-1 α | 41.5 | 0.2 | 4.7 | 8.2 | 9.5 |
| AJ004832 | Neuropathy target esterase | 36.0 | 3.2 | 8.7 | 3.0 | 7.2 |
| U09937 | Urokinase-type plasminogen receptor | 43.6 | 9.3 | 4.6 | 8.6 | 5.8 |
| X70326 | MARCKS-related protein | 42.1 | 2.7 | 10.0 | 10.9 | 5.4 |
| U81375 | Solute carrier family 29 | 29.4 | 10.7 | 0.5 | < 0.1 | 7.9 |
| AB008535 | CRTH2 | 18.2 | 0.6 | 0.4 | < 0.1 | 56.6 |
| M23892 | Arachidonate 15-lipoxygenase | 17.9 | 0.3 | 0.5 | 0.3 | 46.9 |
| AA131149 | S100 calcium-binding protein P | 18.5 | 0.2 | 0.9 | 1.5 | 21.1 |
| AB018339 | Synaptic nuclei expressed gene 1β | 24.3 | 0.8 | 7.3 | 0.4 | 8.5 |
| AF079167 | Lectin-like oxidized LDL receptor | 13.5 | < 0.1 | < 0.1 | 0.2 | 128.9 |
| D13626 | G-protein–coupled receptor for UDP-glucose | 14.2 | 1.1 | 1.0 | 0.7 | 15.0 |
| M34455 | Indoleamine 2,3-dioxygenase | 13.5 | 0.8 | 1.1 | 0.9 | 14.1 |
| X74328 | Cannabinoid receptor 2 | 13.0 | 0.3 | 0.9 | 1.7 | 13.7 |
| L77730 | A3 adenosine receptor | 10.2 | 0.4 | < 0.1 | < 0.1 | 55.5 |
| S59049 | Regulator of G-protein signaling 1 | 12.0 | 2.3 | 1.0 | 0.1 | 10.2 |
| L41816 | CAM kinase I | 10.2 | 1.7 | 1.1 | < 0.1 | 10.7 |
| U21931 | Fructose-1, 6-bisphosphatase 1 | 15.9 | 1.0 | 6.6 | 1.2 | 5.4 |
| U28694 | CCR3 | 16.1 | 0.3 | 1.0 | 8.0 | 5.2 |
| M75914 | IL-5 receptor α chain | 8.8 | 0.7 | 0.7 | 1.1 | 10.5 |
| AF010193 | SMAD7 | 10.9 | 2.2 | 2.4 | 0.2 | 6.7 |
| AF000545 | Purinergic receptor P2Y10 | 14.1 | 0.3 | 3.7 | 4.4 | 5.1 |
| U48250 | Protein kinase C binding protein 2 | 6.2 | 0.1 | 0.1 | 0.4 | 30.4 |
| S68134 | Cyclic AMP-responsive element modulator, β unit | 13.2 | 5.6 | 1.6 | 0.4 | 5.2 |
| L19314 | Phosphorylase kinase, β unit | 7.3 | 1.5 | 0.1 | < 0.1 | 12.7 |
| L08044 | Trefoil factor 3 (intestinal) | 5.6 | < 0.1 | < 0.1 | < 0.1 | 132.9 |
| P1 cre recombinase protein (inner standard 1)2-153 | 131.5 | 81.5 | 106.2 | 97.4 | ||
| P1 cre recombinase protein (inner standard 2) | 109.2 | 62.4 | 79.9 | 56.3 | ||
| Mean of the 6 AD†values | 7759 | 12 071 | 16 498 | 9779 |
| Accession number* . | Name of transcript (protein) . | Eo (%AD†) . | MCs (%AD) . | MNC (%AD) . | Neu (%AD) . | Fold change‡ . |
|---|---|---|---|---|---|---|
| X55988 | Eosinophil-derived neurotoxin | 86.8 | 0.2 | 7.3 | 0.5 | 32.2 |
| L01664 | Charcot-Leyden crystal protein | 78.0 | < 0.1 | 9.2 | 0.2 | 24.5 |
| X81479 | Glycoprotein EMR1 hormone receptor | 59.1 | 1.7 | 5.2 | 2.8 | 18.4 |
| M84526 | Complement factor D | 45.9 | 0.9 | 10.9 | 4.8 | 8.3 |
| J04130 | Macrophage inflammatory protein-1 β | 36.8 | < 0.1 | 6.0 | 1.9 | 14.0 |
| D90144 | Macrophage inflammatory protein-1 α | 41.5 | 0.2 | 4.7 | 8.2 | 9.5 |
| AJ004832 | Neuropathy target esterase | 36.0 | 3.2 | 8.7 | 3.0 | 7.2 |
| U09937 | Urokinase-type plasminogen receptor | 43.6 | 9.3 | 4.6 | 8.6 | 5.8 |
| X70326 | MARCKS-related protein | 42.1 | 2.7 | 10.0 | 10.9 | 5.4 |
| U81375 | Solute carrier family 29 | 29.4 | 10.7 | 0.5 | < 0.1 | 7.9 |
| AB008535 | CRTH2 | 18.2 | 0.6 | 0.4 | < 0.1 | 56.6 |
| M23892 | Arachidonate 15-lipoxygenase | 17.9 | 0.3 | 0.5 | 0.3 | 46.9 |
| AA131149 | S100 calcium-binding protein P | 18.5 | 0.2 | 0.9 | 1.5 | 21.1 |
| AB018339 | Synaptic nuclei expressed gene 1β | 24.3 | 0.8 | 7.3 | 0.4 | 8.5 |
| AF079167 | Lectin-like oxidized LDL receptor | 13.5 | < 0.1 | < 0.1 | 0.2 | 128.9 |
| D13626 | G-protein–coupled receptor for UDP-glucose | 14.2 | 1.1 | 1.0 | 0.7 | 15.0 |
| M34455 | Indoleamine 2,3-dioxygenase | 13.5 | 0.8 | 1.1 | 0.9 | 14.1 |
| X74328 | Cannabinoid receptor 2 | 13.0 | 0.3 | 0.9 | 1.7 | 13.7 |
| L77730 | A3 adenosine receptor | 10.2 | 0.4 | < 0.1 | < 0.1 | 55.5 |
| S59049 | Regulator of G-protein signaling 1 | 12.0 | 2.3 | 1.0 | 0.1 | 10.2 |
| L41816 | CAM kinase I | 10.2 | 1.7 | 1.1 | < 0.1 | 10.7 |
| U21931 | Fructose-1, 6-bisphosphatase 1 | 15.9 | 1.0 | 6.6 | 1.2 | 5.4 |
| U28694 | CCR3 | 16.1 | 0.3 | 1.0 | 8.0 | 5.2 |
| M75914 | IL-5 receptor α chain | 8.8 | 0.7 | 0.7 | 1.1 | 10.5 |
| AF010193 | SMAD7 | 10.9 | 2.2 | 2.4 | 0.2 | 6.7 |
| AF000545 | Purinergic receptor P2Y10 | 14.1 | 0.3 | 3.7 | 4.4 | 5.1 |
| U48250 | Protein kinase C binding protein 2 | 6.2 | 0.1 | 0.1 | 0.4 | 30.4 |
| S68134 | Cyclic AMP-responsive element modulator, β unit | 13.2 | 5.6 | 1.6 | 0.4 | 5.2 |
| L19314 | Phosphorylase kinase, β unit | 7.3 | 1.5 | 0.1 | < 0.1 | 12.7 |
| L08044 | Trefoil factor 3 (intestinal) | 5.6 | < 0.1 | < 0.1 | < 0.1 | 132.9 |
| P1 cre recombinase protein (inner standard 1)2-153 | 131.5 | 81.5 | 106.2 | 97.4 | ||
| P1 cre recombinase protein (inner standard 2) | 109.2 | 62.4 | 79.9 | 56.3 | ||
| Mean of the 6 AD†values | 7759 | 12 071 | 16 498 | 9779 |
MARCKS indicates myristoylated alanine-rich C kinase substrate; LDL, low-density lipoprotein; UDP, uridine 5′-diphosphate; CAM kinase, calmodulin-dependent protein kinase.
The GenBank accession number (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide) of each transcript is shown.
The level of gene expression was obtained as the AD using Genechip software. The percentages of the specific AD level versus the mean AD level of 6 probe sets for housekeeping genes (β-actin and glyceraldehyde-3-phosphate dehydrogenase) were then calculated. The mean percent AD levels obtained from cultured eosinophils (Eo, n = 2), PB-derived MCs (n = 6), mononuclear cells (MNC, n = 4), and neutrophils (Neu, n = 1) are shown.
The fold change was obtained by dividing the eosinophil value (Eo) by the mean of the 3 other transcript levels (MCs, MNC, and Neu). The transcripts showing at least a 5-fold change are listed in the table.
Inner standards such as P1 cre recombinase protein were added to cRNA of the target sample just before the hybridization (see “Materials and methods”) to evaluate the difference between samples. The inner standards were found at similar levels (< 2-fold change) after compensation with the housekeeping genes.