Table 1. Oligonucleotide primers used... | American Society of Hematology

Table 1.

Oligonucleotide primers used for Unique Site Elimination site-directed mutagenesis

Name	Sequence	Position
SDM C2008Y	5′-GTG CAG GAC CAG TAC TCC TGC TGC TC-3′	8549-8574
		(in vWF cDNA)
SDM C2008S	5′-CAG GAC CAGAGC TCC TGC TGC TCT-3′	8552-8575
		(in vWF cDNA)
SDMpSP70HpaI	5′-CGA TAA GCC AGG GTACCC TGC ATT AAT GAA TCG-3′	78-110
		(in pSP70)

Name	Sequence	Position
SDM C2008Y	5′-GTG CAG GAC CAG TAC TCC TGC TGC TC-3′	8549-8574
		(in vWF cDNA)
SDM C2008S	5′-CAG GAC CAGAGC TCC TGC TGC TCT-3′	8552-8575
		(in vWF cDNA)
SDMpSP70HpaI	5′-CGA TAA GCC AGG GTACCC TGC ATT AAT GAA TCG-3′	78-110
		(in pSP70)

Underlined nucleotides indicate base substitutions.