Correlation of expression patterns between mRNA level and protein level
| Spot . | Protein definition . | Gi number . | Predicted value . | Percentage (%) . | 2DE pattern . | cDNA expression pattern . | Ag . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| kd . | pI . | 0 h . | 72 h . | 0 h . | 72 h . | |||||
| 1 | GRP 78 | 2506545 | 72.4 | 5.1 | 1 | 3 | 1321 | 1043.3 | N | |
| 2 | Actin, gamma, cytoplasmic | 6752954 | 41.77 | 5.3 | 40 | 3 | 6 | 0 | 2 | Y |
| 3 | RHO GDI 2 | 2494703 | 22.83 | 4.9 | 33 | 3 | 3 | 341 | 441.6 | Y |
| 4 | Proliferating cell nuclear antigen | 7242171 | 28.77 | 4.7 | 42 | 1 | 0 | 544 | 430.9 | Y |
| 5 | APS kinase | 4038346 | 69.8 | 7.1 | 24 | 2 | 1 | 43 | 50.7 | N |
| 6 | Pyruvate kinase 3 | 6755074 | 57.9 | 7.2 | 48 | 6 | 4 | 3047 | 5880.3 | N |
| 7 | Melanoma X-actin | 6671509 | 41.72 | 5.3 | 39 | 1 | 3 | 2539 | 341.3 | N |
| 8 | Glyceraldehyde-3-phosphate dehydrogenase | 6679937 | 35.79 | 8.7 | 39 | 8 | 7 | 3073 | 5742.3 | N |
| 9 | Stefin 3 | 461911 | 10.99 | 5.9 | 48 | 0 | 4 | N/A | N/A | — |
| 10 | Guanine nucleotide binding protein, beta-2, related sequence1 | 6680047 | 35.06 | 7.9 | 21 | 4 | 2 | 139 | 303.1 | N |
| 11 | Triosephosphate isomerase | 6678413 | 26.69 | 6.9 | 26 | 3 | 3 | 3312 | 2660.1 | Y |
| 12 | Testis-derived c-abl protein | 1196524 | 17.19 | 7 | 51 | 2 | 3 | 152 | 126.9 | N |
| 13 | RNA binding motif protein 3 | 7949121 | 16.59 | 6.8 | 25 | 1 | 0 | 628 | 812.4 | N |
| 14 | Collapsin response mediator | 6681019 | 62.16 | 6.4 | 36 | 2 | 0 | Absent | Absent | N |
| 15 | Lamin A | 220474 | 47.52 | 6.6 | 35 | 2 | 0 | Absent | Absent | N |
| 16 | 47-kd keratin | 52783 | 35.82 | 4.8 | 29 | 3 | 0 | Absent | Absent | N |
| 17 | sid478p | 5931565 | 31.3 | 6.7 | 30 | 1 | 2 | Absent | Absent | N |
| 18 | MHC class II H2-IA-beta-5 | 3169662 | 28.6 | 7.1 | 39 | 1 | 2 | N/A | N/A | — |
| 19 | Androgen-binding protein: subunit alpha | 739346 | 8.04 | 6.4 | 68 | 0 | 2 | Absent | Absent | N |
| 20 | Neuronal apoptosis inhibitory protein | 5932010 | 158.7 | 6 | 17 | 1 | 0 | N/A | N/A | — |
| 21 | PAD type IV | 6755018 | 74.46 | 7.2 | 21 | 1 | 3 | N/A | N/A | — |
| 22 | Human serum albumin homologoue | 3212625 | 66.45 | 5.7 | 24 | 0 | 6 | N/A | N/A | — |
| 23 | syncrip | 6576815 | 62.53 | 7.2 | 33 | 2 | 1 | N/A | N/A | — |
| 24 | Transamidinase | 1730203 | 48.22 | 7.2 | 31 | 3 | 1 | N/A | N/A | — |
| 25 | PGK crigr phosphoglycerate | 1730519 | 44.54 | 8.3 | 47 | 5 | 4 | 1088 | 1402.3 | N |
| 26 | Proliferation-associated gene A | 6754976 | 22.16 | 8.6 | 53 | 3 | 1 | N/A | N/A | — |
| 27 | Putatuve peroxisomal antioxidant enzyme | 3913065 | 17 | 7.8 | 55 | 0 | 3 | N/A | N/A | — |
| 28 | IgE chain C2 region | 2137430 | 12.1 | 5.2 | 38 | 0 | 1 | N/A | N/A | — |
| Spot . | Protein definition . | Gi number . | Predicted value . | Percentage (%) . | 2DE pattern . | cDNA expression pattern . | Ag . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| kd . | pI . | 0 h . | 72 h . | 0 h . | 72 h . | |||||
| 1 | GRP 78 | 2506545 | 72.4 | 5.1 | 1 | 3 | 1321 | 1043.3 | N | |
| 2 | Actin, gamma, cytoplasmic | 6752954 | 41.77 | 5.3 | 40 | 3 | 6 | 0 | 2 | Y |
| 3 | RHO GDI 2 | 2494703 | 22.83 | 4.9 | 33 | 3 | 3 | 341 | 441.6 | Y |
| 4 | Proliferating cell nuclear antigen | 7242171 | 28.77 | 4.7 | 42 | 1 | 0 | 544 | 430.9 | Y |
| 5 | APS kinase | 4038346 | 69.8 | 7.1 | 24 | 2 | 1 | 43 | 50.7 | N |
| 6 | Pyruvate kinase 3 | 6755074 | 57.9 | 7.2 | 48 | 6 | 4 | 3047 | 5880.3 | N |
| 7 | Melanoma X-actin | 6671509 | 41.72 | 5.3 | 39 | 1 | 3 | 2539 | 341.3 | N |
| 8 | Glyceraldehyde-3-phosphate dehydrogenase | 6679937 | 35.79 | 8.7 | 39 | 8 | 7 | 3073 | 5742.3 | N |
| 9 | Stefin 3 | 461911 | 10.99 | 5.9 | 48 | 0 | 4 | N/A | N/A | — |
| 10 | Guanine nucleotide binding protein, beta-2, related sequence1 | 6680047 | 35.06 | 7.9 | 21 | 4 | 2 | 139 | 303.1 | N |
| 11 | Triosephosphate isomerase | 6678413 | 26.69 | 6.9 | 26 | 3 | 3 | 3312 | 2660.1 | Y |
| 12 | Testis-derived c-abl protein | 1196524 | 17.19 | 7 | 51 | 2 | 3 | 152 | 126.9 | N |
| 13 | RNA binding motif protein 3 | 7949121 | 16.59 | 6.8 | 25 | 1 | 0 | 628 | 812.4 | N |
| 14 | Collapsin response mediator | 6681019 | 62.16 | 6.4 | 36 | 2 | 0 | Absent | Absent | N |
| 15 | Lamin A | 220474 | 47.52 | 6.6 | 35 | 2 | 0 | Absent | Absent | N |
| 16 | 47-kd keratin | 52783 | 35.82 | 4.8 | 29 | 3 | 0 | Absent | Absent | N |
| 17 | sid478p | 5931565 | 31.3 | 6.7 | 30 | 1 | 2 | Absent | Absent | N |
| 18 | MHC class II H2-IA-beta-5 | 3169662 | 28.6 | 7.1 | 39 | 1 | 2 | N/A | N/A | — |
| 19 | Androgen-binding protein: subunit alpha | 739346 | 8.04 | 6.4 | 68 | 0 | 2 | Absent | Absent | N |
| 20 | Neuronal apoptosis inhibitory protein | 5932010 | 158.7 | 6 | 17 | 1 | 0 | N/A | N/A | — |
| 21 | PAD type IV | 6755018 | 74.46 | 7.2 | 21 | 1 | 3 | N/A | N/A | — |
| 22 | Human serum albumin homologoue | 3212625 | 66.45 | 5.7 | 24 | 0 | 6 | N/A | N/A | — |
| 23 | syncrip | 6576815 | 62.53 | 7.2 | 33 | 2 | 1 | N/A | N/A | — |
| 24 | Transamidinase | 1730203 | 48.22 | 7.2 | 31 | 3 | 1 | N/A | N/A | — |
| 25 | PGK crigr phosphoglycerate | 1730519 | 44.54 | 8.3 | 47 | 5 | 4 | 1088 | 1402.3 | N |
| 26 | Proliferation-associated gene A | 6754976 | 22.16 | 8.6 | 53 | 3 | 1 | N/A | N/A | — |
| 27 | Putatuve peroxisomal antioxidant enzyme | 3913065 | 17 | 7.8 | 55 | 0 | 3 | N/A | N/A | — |
| 28 | IgE chain C2 region | 2137430 | 12.1 | 5.2 | 38 | 0 | 1 | N/A | N/A | — |
The proteins listed here are represented by the spots marked in the electrophoretograms shown in Figure 5.
Protein definition, Gi number, and predicted value refer to the protein name, accession number, and properties derived from the National Center for Biotechnology Information protein database. The column labeled % shows the percentage of peptides predicted from the protein sequence that were detected by mass spectroscopy. The expression level of protein spots expressed in mouse promyelocytic cell line cell induced by all-trans retinoic acid for 0 hours and 72 hours (Figure5) were scored on a scale of 1 (+) to 8 (++++++++) in the 2DE pattern column. The cDNA expression patterns of the cognate mRNAs are listed in the cDNA expression pattern column abstracted from the dbMC database. The genes not represented on the oligonucleotide arrays were marked as N/A. Ag showed the correlation of gene patterns at mRNA level or protein level.
Y indicates agreement and N discrepancy between changes in cDNA and protein spot intensity. The numbers in bold were obtained with DD. 2DE indicates 2-dimensional gel electrophoresis; IgE, immunoglobulin E; DD, differential display.