Interleukin-6R subunits gp80 and gp130 are expressed in Hodgkin cell lines
| . | gp130 . | gp130 . | gp80 . | IL-6 . |
|---|---|---|---|---|
| Cell line . | (immunoblot) . | (FACS analysis) . | (pg/mL)* . | |
| U266 | ++++ | 5.8 | 29 | 34 |
| L428 | − | 0.2 | 0.6 | 260 |
| L540 | ++ | 0.8 | 3.1 | 15 |
| L591 | ++ | 1.1 | 1.6 | 570 |
| L1236 | − | 2.5 | 1.3 | 620 |
| KMH2 | ++++ | 7.1 | 0 | 122 |
| HDLM2 | − | 1.5 | 10.5 | 146 |
| Dev | + | 0.8 | 0.5 | 23 |
| . | gp130 . | gp130 . | gp80 . | IL-6 . |
|---|---|---|---|---|
| Cell line . | (immunoblot) . | (FACS analysis) . | (pg/mL)* . | |
| U266 | ++++ | 5.8 | 29 | 34 |
| L428 | − | 0.2 | 0.6 | 260 |
| L540 | ++ | 0.8 | 3.1 | 15 |
| L591 | ++ | 1.1 | 1.6 | 570 |
| L1236 | − | 2.5 | 1.3 | 620 |
| KMH2 | ++++ | 7.1 | 0 | 122 |
| HDLM2 | − | 1.5 | 10.5 | 146 |
| Dev | + | 0.8 | 0.5 | 23 |
Expression of gp130 was evaluated by flow cytometry and Western blot analysis; gp80 staining was evaluated by flow cytometry. The data are presented as mean fluorescence. IL-6 expression was measured by ELISA.
FACS indicates fluorescence-activated cell sorting; IL-6, interleukin-6; ELISA, enzyme-linked immunosorbent assay.
Detection limit of the ELISA was 3.5 pg/mL.