Comparison of malignancies with a CD3−/CD56+/CD57− phenotype and leukemic presentation
| . | Patient's tumor . | Myeloid/NK cell acute leukemia . | Myeloid/NK cell precursor acute leukemia . | Blastic NK cell lymphoma/leukemia . | Aggressive NK cell leukemia . |
|---|---|---|---|---|---|
| Phenotype | CD7−, CD13+, CD30+, CD15−, CD33+/−, CD34−, HLA-DR+ | CD33+, CD16−, CD34+/−, HLA-DR− | CD7+, CD33 or CD13+; CD34+; CD16−; CD15+/−; HLA-DR+/− | CD4+/−, CD16−, CD33−, TdT(IHC)+/−, HLA-DR+ | CD2+, CD5−, CD16+/−, CD33−, HLA-DR+ |
| Cytochemical MPO reactivity | Negative | Positive | Negative | Negative | Negative |
| Cytoplasmic expression of MPO by IHC | Positive | Unknown | Frequently positive | Negative | Negative |
| Morphology | Monocytoid | Promyelocytoid | Immature blastoid | Lymphoblastoid | Large granular lymphocytes |
| Granzyme B | Positive | Negative | Negative | Negative | Positive |
| Functional NK cytotoxicity | Present | Present only following IL-2 stimulation | Absent | Absent | Present |
| IL-2R (CD25) | Positive | Unknown | Negative | Negative | Positive |
| Bone marrow involvement | Secondarily involved | Always | Occasional | Rare | Common |
| Extramedullary disease | Present | Uncommon | Common | Occasional | Common |
| Lymph node involvement | Not detected | Common | Common | Occasional | Common |
| TCR rearrangement | Germline | Primarily germline | Primarily germline | Primarily germline | Germline |
| . | Patient's tumor . | Myeloid/NK cell acute leukemia . | Myeloid/NK cell precursor acute leukemia . | Blastic NK cell lymphoma/leukemia . | Aggressive NK cell leukemia . |
|---|---|---|---|---|---|
| Phenotype | CD7−, CD13+, CD30+, CD15−, CD33+/−, CD34−, HLA-DR+ | CD33+, CD16−, CD34+/−, HLA-DR− | CD7+, CD33 or CD13+; CD34+; CD16−; CD15+/−; HLA-DR+/− | CD4+/−, CD16−, CD33−, TdT(IHC)+/−, HLA-DR+ | CD2+, CD5−, CD16+/−, CD33−, HLA-DR+ |
| Cytochemical MPO reactivity | Negative | Positive | Negative | Negative | Negative |
| Cytoplasmic expression of MPO by IHC | Positive | Unknown | Frequently positive | Negative | Negative |
| Morphology | Monocytoid | Promyelocytoid | Immature blastoid | Lymphoblastoid | Large granular lymphocytes |
| Granzyme B | Positive | Negative | Negative | Negative | Positive |
| Functional NK cytotoxicity | Present | Present only following IL-2 stimulation | Absent | Absent | Present |
| IL-2R (CD25) | Positive | Unknown | Negative | Negative | Positive |
| Bone marrow involvement | Secondarily involved | Always | Occasional | Rare | Common |
| Extramedullary disease | Present | Uncommon | Common | Occasional | Common |
| Lymph node involvement | Not detected | Common | Common | Occasional | Common |
| TCR rearrangement | Germline | Primarily germline | Primarily germline | Primarily germline | Germline |
NK, natural killer; IHC, immunohistochemistry; MPO, myeloperoxidase; IL-2, interleukin 2; IL-2R, interleukin-2 receptor; TCR, T-cell receptor.