TP53 primers used for polymerase chain reaction and sequencing
| Name . | Sequence 5′-3′ . | nt numbers X54156 . |
|---|---|---|
| R1F | AGCGTGCTTTCCACGACGGTG | 910-930 (S) |
| 2SQF | TGGATTGGCAGCCAGACTG | 942-949, 11689-11699 (S) |
| 4F | CAACGTTCTGGTAAGGAC | 11918-11935 (S) |
| 4SQF | GAAAACCTACCAGGGCAG | 12224-12241 (S) |
| 4SQB | GTCACAGACTTGGCTGTC | 12275-12292 (AS) |
| 4SQB2 | AAGCCTAAGGGTGAAGTG | 12388-12405 (AS) |
| 4B | ACAGGAAGCCTAAGGGTGAAGAG | 12388-12410 (AS) |
| 5/6F | TGCTGCCGTGTTCCAGTTGC | 12979-12997 (S) |
| 5SQF | ATCTGTTCACTTGTGCCCTG | 13002-13021 (S) |
| 5SQB | ATCAACCCACAGCTGCAC | 13106-13123 (AS) |
| 6SQF | TGAGCAGCTGGGGCTGGAGAG | 13240-13260 (S) |
| 5/6B | GGAGGGCCACTGACAACCAC | 13474-13493 (AS) |
| 7/8F | GAAGCTTACGAGGCTAAGG | 13599-13617 (S) |
| 7SQF | CTGCTTGCCACAGGTCTCC | 13938-13956 (S) |
| 7SQF2 | CTCACCATCATCACACTG | 14081-14098 (S) |
| 8SQF | TGGGACAGGTAGGACCTG | 14391-14408 (S) |
| 8SQB | GCGGAGATTCTCTTCCTC | 14522-14539 (AS) |
| 7/8B | GCTGGTGTTGTTGGGCAG | 14683-14700 (AS) |
| 9F | CACTAAGCGAGGTAAGCAAGCAG | 14578-14600 (S) |
| 9SQB | GTCTTTGAGGCATCACTG | 14866-14883 (AS) |
| 9B | AGCTACAACCAGGAGCCATTGTC | 14881-14903 (AS) |
| 10F | GCTTTTGATCCGTCATAAAGTC | 17472-17493 (S) |
| 10SQB | AGGATGAGAATGGAATCC | 17740-17757 (AS) |
| 10B | GCTGCCTTTGACCATGAAGGCAG | 17756-17778 (AS) |
| 11SQB | ACGCACACCTATTGCAAG | 18770-18787 (AS) |
| R11B | CTGGGTGCTTCTGACGCACAC | 18780-18800 (AS) |
| Name . | Sequence 5′-3′ . | nt numbers X54156 . |
|---|---|---|
| R1F | AGCGTGCTTTCCACGACGGTG | 910-930 (S) |
| 2SQF | TGGATTGGCAGCCAGACTG | 942-949, 11689-11699 (S) |
| 4F | CAACGTTCTGGTAAGGAC | 11918-11935 (S) |
| 4SQF | GAAAACCTACCAGGGCAG | 12224-12241 (S) |
| 4SQB | GTCACAGACTTGGCTGTC | 12275-12292 (AS) |
| 4SQB2 | AAGCCTAAGGGTGAAGTG | 12388-12405 (AS) |
| 4B | ACAGGAAGCCTAAGGGTGAAGAG | 12388-12410 (AS) |
| 5/6F | TGCTGCCGTGTTCCAGTTGC | 12979-12997 (S) |
| 5SQF | ATCTGTTCACTTGTGCCCTG | 13002-13021 (S) |
| 5SQB | ATCAACCCACAGCTGCAC | 13106-13123 (AS) |
| 6SQF | TGAGCAGCTGGGGCTGGAGAG | 13240-13260 (S) |
| 5/6B | GGAGGGCCACTGACAACCAC | 13474-13493 (AS) |
| 7/8F | GAAGCTTACGAGGCTAAGG | 13599-13617 (S) |
| 7SQF | CTGCTTGCCACAGGTCTCC | 13938-13956 (S) |
| 7SQF2 | CTCACCATCATCACACTG | 14081-14098 (S) |
| 8SQF | TGGGACAGGTAGGACCTG | 14391-14408 (S) |
| 8SQB | GCGGAGATTCTCTTCCTC | 14522-14539 (AS) |
| 7/8B | GCTGGTGTTGTTGGGCAG | 14683-14700 (AS) |
| 9F | CACTAAGCGAGGTAAGCAAGCAG | 14578-14600 (S) |
| 9SQB | GTCTTTGAGGCATCACTG | 14866-14883 (AS) |
| 9B | AGCTACAACCAGGAGCCATTGTC | 14881-14903 (AS) |
| 10F | GCTTTTGATCCGTCATAAAGTC | 17472-17493 (S) |
| 10SQB | AGGATGAGAATGGAATCC | 17740-17757 (AS) |
| 10B | GCTGCCTTTGACCATGAAGGCAG | 17756-17778 (AS) |
| 11SQB | ACGCACACCTATTGCAAG | 18770-18787 (AS) |
| R11B | CTGGGTGCTTCTGACGCACAC | 18780-18800 (AS) |
Nucleotide (nt) numbers correspond to the genomic sequence, accession number X54156. S indicates sense strand; AS, antisense strand. Primer 2SQF is derived from exons 1 and 2.