Summary of cytotoxicity assays for CMV peptide–induced CTL lines
| Culture conditions . | No. lines . | No. of CMV-specific lines . | Lysis of CMV peptide–pulsed targets at 1:1 range (average) . | Nonspecific lysis at 1:1 of control targets . | Lysis of CMV peptide–pulsed targets at 1:5 range (average) . | Nonspecific lysis at 1:5 of control targets . | Lysis of CMV peptide–pulsed targets at 1:10 range (average) . | Nonspecific lysis at 1:10 of control targets . |
|---|---|---|---|---|---|---|---|---|
| CD8+ | 5 | 4 (80%) | 17-22% (20%) | < 3% | 59% | < 1% | 66-70% (68%) | 10% |
| CD8+, IL-7+ | 9 | 9 (100%) | 5-54% (22%) | < 1% | 18-96% (57%) | < 1% | 31-93% (62%) | < 3% |
| CD14− | 2 | 2 (100%) | 4-5% (5%) | < 1% | 16% | — | 27% (27%) | 9% |
| CD14−, IL-7+ | 3 | 2 (67%) | 4-7% (6%) | < 1% | — | — | 26-29% (28%) | < 6% |
| Total | 19 | 17 (89%) | 4-50% (11%) | < 1-3% | 16-96% (50%) | — | 26-93% (52%) | < 3-10% |
| Culture conditions . | No. lines . | No. of CMV-specific lines . | Lysis of CMV peptide–pulsed targets at 1:1 range (average) . | Nonspecific lysis at 1:1 of control targets . | Lysis of CMV peptide–pulsed targets at 1:5 range (average) . | Nonspecific lysis at 1:5 of control targets . | Lysis of CMV peptide–pulsed targets at 1:10 range (average) . | Nonspecific lysis at 1:10 of control targets . |
|---|---|---|---|---|---|---|---|---|
| CD8+ | 5 | 4 (80%) | 17-22% (20%) | < 3% | 59% | < 1% | 66-70% (68%) | 10% |
| CD8+, IL-7+ | 9 | 9 (100%) | 5-54% (22%) | < 1% | 18-96% (57%) | < 1% | 31-93% (62%) | < 3% |
| CD14− | 2 | 2 (100%) | 4-5% (5%) | < 1% | 16% | — | 27% (27%) | 9% |
| CD14−, IL-7+ | 3 | 2 (67%) | 4-7% (6%) | < 1% | — | — | 26-29% (28%) | < 6% |
| Total | 19 | 17 (89%) | 4-50% (11%) | < 1-3% | 16-96% (50%) | — | 26-93% (52%) | < 3-10% |
CTL lines capable of killing CMV peptide-pulsed targets were produced in 17 of 19 cultures derived from seropositive donors. Cultures were initiated with purified CD8+ T cells or PBMCs depleted of CD14+ cells. In general, CTL lines generated from purified CD8+ cells achieved higher specific lysis of targets than CD14− lines. This is probably due to the presence of expanded nonspecific CD4+ and CD56+cell populations in the CD14− cultures.