Numbers of donor-derived cells in the secondary recipients 4 weeks after intravenous transfer of thymus cells from primary recipients
| Days after primary BM transfer . | Thymus (× 103) . | Spleen and lymph nodes (× 103) . | BM (× 103) . |
|---|---|---|---|
| 1 | 11 | 37 | 19 |
| 2 | 10 | 46 | > 1 |
| 3 | 14 | 62 | 1 |
| 4 | 14 | 366 | 12 |
| 5 | 10 | 70 | 8 |
| 6 | 416 | 580 | 23 |
| Days after primary BM transfer . | Thymus (× 103) . | Spleen and lymph nodes (× 103) . | BM (× 103) . |
|---|---|---|---|
| 1 | 11 | 37 | 19 |
| 2 | 10 | 46 | > 1 |
| 3 | 14 | 62 | 1 |
| 4 | 14 | 366 | 12 |
| 5 | 10 | 70 | 8 |
| 6 | 416 | 580 | 23 |
Cell suspensions were made from thymus, spleen plus lymph nodes, and BM of the secondary recipients 4 weeks after IV transfer of thymus cells obtained from the primary recipients 1 to 6 days after initial BM transfer. Suspensions were then stained for donor-type Ly-5.2. The average number of donor-derived cells per organ was calculated by multiplying the total cell number of each organ by the proportion of Ly-5.2+ cells. BM cells were collected from femurs and tibias of each secondary recipient mouse. Results shown in this table are representative of 3 such experiments for each time point. Each experiment included 2 to 3 recipient mice.
BM indicates bone marrow; IV, intravenous.