Mean fluorescence intensity of Alexa 488IB5 IgG binding to mouse platelets isolated before or at various times after in vivo administration of 1B5 Fab
| Time after injection . | Alexa 488IB5 IgG mean fluorescence intensity . | |
|---|---|---|
| 1B5 Fab 0.53 mg/kg IV . | 1B5 Fab 1 mg/kg IV . | |
| Baseline | 3.30 | 3.10 |
| 7 minutes | 0.68 | ND |
| 1 hour | 0.78 | 0.02 |
| 5 hours | ND | 0.04 |
| 18 hours | ND | 0.60 |
| 24 hours | 1.50 | ND |
| 5 days | 3.10 | ND |
| Time after injection . | Alexa 488IB5 IgG mean fluorescence intensity . | |
|---|---|---|
| 1B5 Fab 0.53 mg/kg IV . | 1B5 Fab 1 mg/kg IV . | |
| Baseline | 3.30 | 3.10 |
| 7 minutes | 0.68 | ND |
| 1 hour | 0.78 | 0.02 |
| 5 hours | ND | 0.04 |
| 18 hours | ND | 0.60 |
| 24 hours | 1.50 | ND |
| 5 days | 3.10 | ND |
Results are from 2 individual mice. Similar results were obtained through the first 6 hours after injection in 2 additional mice receiving 1B5 Fab (1 mg/kg, IP) and 4 mice receiving 1B5 F(ab′)2 (1-2 mg/kg, IP).
ND, not determined.