Binding of anti–glycoprotein Ib monoclonal antibodies to the canine-human and the rehumanized chimeras
| Canine-human chimera . | Group 1 . | Group 2 . | |||
|---|---|---|---|---|---|
| 27A10 . | 12G1 . | 12E4 . | 6B4 . | 24G10 . | |
| C35 | 93 | 2 | 3 | 98 | 2 |
| C59 | 8 | 4 | 4 | 92 | 4 |
| C81 | 2 | 2 | 2 | 98 | 2 |
| C104 | 3 | 3 | 3 | 99 | 3 |
| C152 | 2 | 2 | 2 | 87 | 2 |
| C200 | 2 | 2 | 2 | 97 | 1 |
| C268 | 2 | 2 | 2 | 3 | 3 |
| C282 | 4 | 4 | 4 | 4 | 4 |
| Rehumanized chimera | |||||
| H59 | 84 | 78 | 81 | 8 | 4 |
| H81 | 75 | 73 | 76 | 6 | 73 |
| H104 | 98 | 97 | 99 | 6 | 96 |
| H128 | 72 | 73 | 74 | 5 | 81 |
| H152 | 97 | 98 | 98 | 4 | 96 |
| H176 | 95 | 96 | 97 | 8 | 89 |
| Canine-human chimera . | Group 1 . | Group 2 . | |||
|---|---|---|---|---|---|
| 27A10 . | 12G1 . | 12E4 . | 6B4 . | 24G10 . | |
| C35 | 93 | 2 | 3 | 98 | 2 |
| C59 | 8 | 4 | 4 | 92 | 4 |
| C81 | 2 | 2 | 2 | 98 | 2 |
| C104 | 3 | 3 | 3 | 99 | 3 |
| C152 | 2 | 2 | 2 | 87 | 2 |
| C200 | 2 | 2 | 2 | 97 | 1 |
| C268 | 2 | 2 | 2 | 3 | 3 |
| C282 | 4 | 4 | 4 | 4 | 4 |
| Rehumanized chimera | |||||
| H59 | 84 | 78 | 81 | 8 | 4 |
| H81 | 75 | 73 | 76 | 6 | 73 |
| H104 | 98 | 97 | 99 | 6 | 96 |
| H128 | 72 | 73 | 74 | 5 | 81 |
| H152 | 97 | 98 | 98 | 4 | 96 |
| H176 | 95 | 96 | 97 | 8 | 89 |
Binding was analyzed using fluorescein isothiocyanate–labeled secondary antibody and flow cytometry. Binding is expressed as percentage positive cells.