Fibrin(ogen) P1 and P2 exposure on plasma-coated and fibrinogen-coated polyethylene terephthalate disks and fibrin-coated glass beads (n = 4 in all cases)
| Sample coating . | First antibody buffer . | P1 . | P2 . |
|---|---|---|---|
| (OD/cm2) . | |||
| Albumin-PET | PBS | 0.000 ± 0.005 | 0.000 ± 0.003 |
| Fibrinogen-PET | PBS | 0.105 ± 0.023 | 0.196 ± 0.030 |
| 1 mg/mL fibrinogen* | 0.109 ± 0.009 | 0.190 ± 0.041 | |
| 1 mg/mL P1 or P2† | 0.003 ± 0.002 | 0.011 ± 0.005 | |
| Plasma-PET | PBS | 0.040 ± 0.010 | 0.059 ± 0.015 |
| Fibrin-glass | PBS | 0.630 ± 0.046 | 0.493 ± 0.066 |
| Sample coating . | First antibody buffer . | P1 . | P2 . |
|---|---|---|---|
| (OD/cm2) . | |||
| Albumin-PET | PBS | 0.000 ± 0.005 | 0.000 ± 0.003 |
| Fibrinogen-PET | PBS | 0.105 ± 0.023 | 0.196 ± 0.030 |
| 1 mg/mL fibrinogen* | 0.109 ± 0.009 | 0.190 ± 0.041 | |
| 1 mg/mL P1 or P2† | 0.003 ± 0.002 | 0.011 ± 0.005 | |
| Plasma-PET | PBS | 0.040 ± 0.010 | 0.059 ± 0.015 |
| Fibrin-glass | PBS | 0.630 ± 0.046 | 0.493 ± 0.066 |
OD indicates optical density; PET, polyethylene terephthalate; PBS, phosphate-buffered saline.
Soluble fibrinogen (1 mg/mL) was added to test whether soluble fibrinogen possesses P1 or P2 epitope.
Soluble P1 and P2 peptides (1 mg/mL) were added in enzyme-linked immunosorbent assays to test the specificity of P1 and P2 antibodies.